沙芥果皮浸提液对白菜种子化感作用及其化感物质分析

doi:10.3969/j.issn.2096-1197.2019.04.02

Abstract

Abstract: The effects of the extraction of methanol phase,ethyl acetate phase,ethyl ether phase,petroleum ether phase and water phase of water-soaked peels of Pugionium cornutum （L.） Gaertn on the germination rates and growth of cabbage were investigated through the bioassay method,and the chemical compositions of the extracts were isolated and identified by GC-MS.The results showed that Pugionium cornutum （L.） Gaertn peels extracts inhibited cabbage seed germination and seedling growth,with the inhibitory effect in proportion to the extract concentration.Allelopathy comprehensive effects of the organic phases with different concentrations were between -0.01 and -0.49.At the same concentration,the inhibitory effects of the extracts of Pugionium cornutum （L.） Gaertn peels on the seed germination and seedling growth were：ethyl ether phase > ethyl acetate phase > methanol > water phase > petroleum ether phase,indicating that ethyl ether phase was the superior phase.Main organic compounds were identified in organic extracts from peels of Pugionium cornutum （L.） Gaertn,including phenolic aciods,organic acidr,alcohol,arenes,of which 1,2-Benzenedicarboxylic acid 1H-Indene and 15-Crown-5 were the common material of all organic phase.Phthalic acid and 15-crown ether-5 were the common substances of each phase.The extracts of each isolated phase with > 0.75 g/mL concentration had strong inhibitory effects on the germination of cabbage seeds,the seedling height,and root length.

Key words: Pugionium cornutum （L.） Gaertn, Cabbage, Allelopathy, Allelochemicals

CLC Number:

S647

BAO Hongchun, HAO Lizhen, ZHANG Fenglan, YANG Zhongren, LI Xiaolei, ZHENG Qingling. Allelopathy of cabbage seeds soaked from the extraction of peels of Pugionium cornutum （L.）Gaertn and analysis of its Allelochemicals[J].Journal of Northern Agriculture, 2019, 47(4): 8-14.

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Allelopathy of cabbage seeds soaked from the extraction of peels of Pugionium cornutum （L.）Gaertn and analysis of its Allelochemicals

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