果桑离体组织培养快繁体系的建立

doi:10.3969/j.issn.2096-1197.2019.06.23

Abstract

Abstract: In order to establish the tissue culture system of rapid propagation of fruit mulberry,the effects of different plant growth regulators and different concentration ratios on the growth of fruit mulberry tissue culture seedlings were studied by taking the variety of Baiyuwang stem segment with axillary bud as explants,and the optimum axillary bud induction,proliferation and rooting medium was selected.The results showed that the sterilization method of explants was 75% ethanol+sterilization 30 s+0.1% HgCl₂+immersion for 10 min.The optimal medium for axillary bud induction was MS+2.0 mg/L 6-BA+1.5 mg/L IBA.The culture medium was MS+2.5 mg/L 6-BA+0.5 mg/L IBA.The rooting medium was 1/2 ms+0.5 mg/L NAA.It provides the research foundation for the fruit mulberry Baiyuwang seedling in the factory.

Key words: Fruit mulberry, Tissue culture, Axillary bud stem section

CLC Number:

Q943.1

TAN Jun, LUO Guijie, MA Bailin, LIU Bo, YIN Qing. Establishment of the in vitro culture and rapid propagation system of fruit mulberry[J].Journal of Northern Agriculture, 2019, 47(6): 130-134.

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Establishment of the in vitro culture and rapid propagation system of fruit mulberry

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