不同改良措施对黄瓜连作土壤细菌群落结构组成和多样性的影响

doi:10.12190/j.issn.2096-1197.2022.02.04

Abstract

Abstract: 【Objective】The impacts of various treatments on the structure and diversity of soil microbial communities on continuous cucumber cropping were investigated to select appropriate soil improvement measures.【Methods】The soil that had been continuously cropped cucumbers for 12 years was treated with straw returning,wheat filling,anti-continuous cropping biological fertilizer,and lime nitrogen disinfection. The Illumina MiSeq sequencing platform was used to conduct a 16S rRNA high-throughput sequencing of soil microorganisms.【Results】 Compared with the control,Chao1 index,Simpson index of the four treatments all increased,with straw returning and anti-continuous cropping biological fertilizer treatment increased significantly（P<0.05）. At the bacterial phylum level,different treatments had no effect on the community composition. However the relative abundance of Bacteroidetes increased significantly（P<0.05）in the treatments of straw returning,wheat filling,and anti-continuous cropping biological fertilizer. In the treatment of wheat filling and lime nitrogen disinfection,the relative abundance of Actinobacteria and Gemmatimonadetes increased significantly（P<0.05）. In the treatment of anti-continuous cropping biological fertilizer and lime nitrogen disinfection,the relative abundance of Acidobacteria reduced significantly（P<0.05）. At genus level in the four treatment,the relative abundance of dominant bacteria Bifidobacterium was significantly reduced（P<0.05）with the appearence Thermopolyspora and Thermomonospora. In wheat filling,the relative abundance of the dominant bacteria Steroidobacteria increased significantly（P<0.05）. In straw returning and anti-continuous cropping biological fertilizer,the relative abundance of dominant bacteria chryseolinea,cellvibrio and flavisobacter increased significantly（P<0.05）.【Conclusion】In continuous cucumber cultivation,the four improvement measures affected the composition,richness,and diversity of the soil bacterial community. The optimum treatment was adding anti-continuous cropping biological fertilizer,followed by straw returning.

Key words: Treatment measures, Cucumber, Continuous cropping, Soil bacterial community composition, Soil Simpson index

CLC Number:

S642.2

JIANG Wei, XUE Guoping, BAI Hongmei, DU Jinwei, ZHU Chunxia, LI Jie, SONG Qingcheng, JI Shuli, WANG Lisheng. Effects of different treatments on the bacterial community composition and diversity in cucumber continuous cropping soil[J].Journal of Northern Agriculture, 2022, 50(2): 28-37.

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Effects of different treatments on the bacterial community composition and diversity in cucumber continuous cropping soil

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