山药种质资源SSR分析及初级核心种质库的构建

doi:10.12190/j.issn.2096-1197.2022.02.03

Abstract

Abstract: 【Objective】To improve the efficiency of Dioscorea opposita Thunb.（Chinese yam）breeding,the genetic variety of Chinese yam germplasm resources was studied,and the primary core collection was established.【Methods】12 pairs of SSR primers were used to amplify the leaf genomic DNA of 55 Chinese yam germplasm by PCR. Genetic diversity was analyzed. Cluster analysis was used to create the yam primary core collection. 【Results】12 primer pairs amplified 128 loci from 55 Chinese yam germplasm,with a polymorphism ratio of 98.43%. The average number of alleles（Na）at a single locus was 1.984 4. The average number of effective alleles（Ne）was 1.420 4. The average Nei′s gene diversity index（He）was 0.265 0. And the average Shannon-Wiener diversity information index（I） of single material amplified bands was 0.417 2. The genetic similarity coefficient among germplasm was 0.570-0.898 according to cluster analysis. After six times of sampling,it was found that except that the rate of polymorphic loci was significantly lower than that before sampling,the changes of other indexes were small. When the sampling proportion was 30%,the rate of polymorphic loci was 93.75%,which was highly representative and was chosen as the primary core collection of Chinese yam.【Conclusion】The genetic diversity of the 55 Chinese yum material was quite high. The 30% sampled primary core collection could adequately represent the original population.

Key words: Chinese yum（Dioscorea opposita Thunb.）, SSR, Genetic diversity, Primary core collection, Germplasm resources

CLC Number:

S632.1

YANG Fan, ZHANG Yanfang, WANG Jinhua, ZHAO Yuan, ZHAO Lingmin, ZHANG Xiaomeng, ZHANG Yong, HUO Xiuwen. SSR analysis of Chinese yam（Dioscorea opposita Thunb.）germplasm resources and construction of primary core collection[J].Journal of Northern Agriculture, 2022, 50(2): 18-27.

0
/ / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: https://journal30.magtechjournal.com/bfnyxb/EN/10.12190/j.issn.2096-1197.2022.02.03

https://journal30.magtechjournal.com/bfnyxb/EN/Y2022/V50/I2/18

References

[1] 高莉莉,惠富平.中国古代山药栽培历史与利用价值[J].农业考古,2019(1):126-133.
[2] 徐子妍,董凯璇,苏亚东,等.山药的营养功效及加工利用研究进展[J].中国果菜,2019,39(8):52-57.
[3] 国家药典委员会.中华人民共和国药典:一部[M].北京:中国医药科技出版社,2015:28.
[4] 何海玲,单承莺,张卫明,等.山药研究进展[J].中国野生植物资源,2006(6):1-6.
[5] 梁任繁,王军民,覃芳,等.广西山药种质资源聚类分析[J].中国蔬菜,2009(4):30-34.
[6] 许念芳,岳林旭,刘少军,等.山药种质资源的分类及综合分析[J].中国野生植物资源,2019,38(1):54-59.
[7] 李丽,王海岗,张晓丽,等.SSR分子标记在作物遗传育种中的应用[J].山西农业科学,2008,36(3):15-18.
[8] 阮成江,何祯祥,钦佩.中国植物遗传连锁图谱构建研究进展[J].西北植物学报,2002,22(6):246-256.
[9] 廉勇,陈源闽,王勇,等.DNA分子标记技术辅助蔬菜育种研究进展[J].内蒙古农业科技,2008(5):78-79.
[10] 彭斌. 山药基源植物分子鉴别体系建立与珠芽相关基因的转录组分析[D].南京:南京农业大学,2016.
[11] 郭文,李婉琳,肖继坪,等.利用AFLP标记构建4种薯蓣属植物的指纹图谱[J].分子植物育种,2015,13(3):547-555.
[12] KEDRA M O,KASSAHUN T,TEKLEHAIMANOT H.Genetic diversity assessment of yams (Dioscorea spp.) from Ethiopia using inter simple sequence repeat (ISSR) markers[J].African Journal of Biotechnology,2019,18(30):970-977.
[13] BOOY G,HENDRIKS R J J,SMULDERS M J M,et al.Genetic diversity and the survival of populations[J].Plant Biology,2000,2(4):379-395.
[14] 李辛雷,李纪元,范正琪.植物遗传资源核心种质研究进展[J].食品安全质量检测学报,2014,5(11):3733-3738.
[15] 崔洪宇,赵海燕,房文文,等.16个黄瓜杂交种SSR数字指纹图谱的构建[J].分子植物育种,2021,11(1):1-16.
[16] 王郅琪,孙建,梁俊超,等.基于分子标记的江西省芝麻地方种质遗传多样性分析[J].浙江农业学报,2021,33(9):1565-1580.
[17] 李琼,耿臻,杨青春,等.黄淮海50份大豆种质资源SSR遗传多样性分析[J].种子,2021,40(8):39-50.
[18] DUAN Y F,LIU J,XU J F,et al.DNA fingerprinting and genetic diversity analysis with simple sequence repeat markers of 217 potato cultivars (Solanum tuberosum L.) in China[J].American Journal of Potato Research,2019,96(1):12.
[19] SIMONE C,LILIANA R,ISACCO B,et al.Genetic characterization and molecular fingerprint of traditional Umbrian tomato (Solanum lycopersicum L.) landraces through SSR markers and application for varietal identification[J].Genetic Resources and Crop Evolution,2020,67:1807-1820.
[20] 王薇,刘超,崔九成,等.珠子参种质资源遗传多样性的ISSR分析[J].中草药,2014,45(17):2525-2529.
[21] 刘向宇,霍秀文,杨明,等.山药种质资源的ISSR分析及初级核心种质库的构建[J].西北植物学报,2015,35(5):915-921.
[22] 石磊,王萍,杨静,等.籽用西瓜种质资源SSR分析及初级核心种质库构建[J].西北植物学报,2016,36(6):1125-1134.
[23] 高玉坤,崔江慧,项晓冬,等.65个马铃薯品种(系)指纹图谱构建和遗传多样性分析[J].农业生物技术学报,2020,28(8):1363-1378.
[24] 张燕梅,李俊峰,鹿志伟,等.剑麻EST-SSR在丝兰麻和中美麻中的通用性分析[J].热带作物学报,2021,42(7):1824-1830.
[25] 陈占勇,霍秀文,尹春,等.10种长山药花粉形态及分类研究[J].华北农学报,2011,26(5):141-145.
[26] 蔡金辉,严渐子,黄晓辉,等.山药品种资源的分类研究[J].江西农业大学学报,1999(1):55-57.
[27] 李自超,张洪亮,孙传清,等.植物遗传资源核心种质研究现状与展望[J].中国农业大学学报,1999,4(5):51-62.
[28] NYBOM H.Comparison of different nuclear DNA markers for estimating intraspecific genetic diversity in plants[J].Molecular Ecology,2004,13(5):1143-1155.
[29] GU X F,YANG A F,MENG H,et al.In vitro induction of tetraploid plants from diploid Zizyphus jujuba Mill. cv.Zhanhua[J].Plant Cell Reports,2005,24(1):671-676.

SSR analysis of Chinese yam（Dioscorea opposita Thunb.）germplasm resources and construction of primary core collection

PDF (PC)

Like

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Metrics

Comments

Recommended 0

[1]	GUO Chengyu, YI Fengyan, FANG Yongyu, SHI Zhidan, LIU Fang, ZHAO Heping, DING Haijun. Genetic diversity analysis of phenotypic traits in 99 barley germplasm resources [J]. Journal of Northern Agriculture, 2023, 51(4): 1-10.
[2]	CHAI Wenbo, LI Shufen, LI Hongtao, XU Hanyuan, ZHU Qing, WANG Jun. Analysis of Huang-Huai-Hai maize heterotic pattern based on SSR molecular marker technology [J]. Journal of Northern Agriculture, 2022, 50(6): 17-24.
[3]	ZHANG Qi, SUN Rujian, GUO Rongqi, YU Ping, FENG Lei, CHAI Shen, HU Xingguo, SUN Bincheng. Research progress and utilization status of dwarf and density tolerant soybean germplasm resources [J]. Journal of Northern Agriculture, 2022, 50(6): 25-30.
[4]	SUN Pingping, LI Zhengnan, LIU Xin, ZHANG Lei, WANG Yong, FU Chongyi. Resistance evaluation of pepper germplasm resources against pepper Phytophthora and fungicide sensitivity analysis on Phytophthora capsici [J]. Journal of Northern Agriculture, 2022, 50(5): 67-72.
[5]	XIAN Feng, LU Zhanyuan, ZHANG Jianzhong, CHEN Liyu, CHENG Yuchen, YANG Jianqiang, ZHANG Xiangqian, Suhe. Genetic diversity analysis of early maturing cotton germplasm resources base on SSR molecular markers [J]. Journal of Northern Agriculture, 2022, 50(1): 11-17.
[6]	HAN Zhigang, XIE Rui, JIN Xiaolei, GUO Binyu, GUO Jingshan. Genetic diversity analysis of potato germplasm resources based on phenotypic traits [J]. Journal of Northern Agriculture, 2021, 49(5): 9-17.
[7]	GUO Jiang′an, FENG Yong, ZHAO Ruixia, HAN Yuting, SU Erhu, LIANG Hongwei, ZHAO Yiwen, ZHANG Laihou. Analysis of genetic diversity and identification of stem rot resistance in maize backbone inbred lines [J]. Journal of Northern Agriculture, 2021, 49(4): 30-34.
[8]	ZHAO Shangmin, LI Xiaodong, FU Zengjuan, E Yuanyuan, ZHANG Hui, WANG Liang, ZHANG Ziqiang, ZHENG Wenzhe, ZHANG Bizhou, BAI Chen, ZHANG Huizhong. Analysis of genetic diversity and population structure of sugarbeet germplasm resources by SSR markers [J]. Journal of Northern Agriculture, 2021, 49(1): 1-9.
[9]	CUI Guohui, YE Jun, WU Xiaohua, YANG Lei, WANG Xiaobing, TAN Liping, YU Meiling, ZHAO Chunzhi, ZHANG Chunyan, ZHANG Haibin, WU Yunxia, LI Yuanqing. Genetic diversity analysis of phenotype traits of spring wheat parental resources in Inner Mongolia [J]. Journal of Northern Agriculture, 2020, 48(3): 1-9.
[10]	CHEN Wenjin, ZHAO Cunhu, HE Xiaoyong, KONG Qingquan, ZHANG Ziqiang, CHEN Wenhe, YANG Jie. Genetic diversity analysis of agronomic characters of mung bean main cultivars [J]. Journal of Northern Agriculture, 2020, 48(3): 18-25.
[11]	PANG Peng, ZHANG Zhiyong, LI Lijun, QI Bingjie, GUO Zhanbin. Genetic diversity analysis of agronomic characters of quinoa germplasm resources [J]. Journal of Northern Agriculture, 2020, 48(3): 26-31.
[12]	YE Jun, WU Xiaohua, LI Yuanqing, CUI Guohui, WANG Xiaobin, ZHAO Chunzhi, YANG Lei, ZHANG Sanfen, ZHANG Haibin, YU Meiling. Genetic diversity analysis of phenotypic traits in quinoa germplasm resources [J]. Journal of Northern Agriculture, 2020, 48(1): 1-6.
[13]	ZHANG Rui, TANG Aijia, XIE Jihong, SHI Fengling, ZHAO Yan. DNA fingerprinting and genetic diversity analysis of Elymus pastures [J]. Journal of Northern Agriculture, 2019, 47(5): 1-8.
[14]	WANG Shanshan, ZHOU Changyan, ZHANG Xiangqian, LU Zhanyuan, YAN Jingbin, HAO Jianxi, CHENG Yuchen, ZHANG Chunyan. Yield difference analysis and genetic diversity of the main agronomic traits in aerospace breeding wheat strains [J]. Journal of Northern Agriculture, 2019, 47(3): 1-6.
[15]	CHEN Wenjin, KONG Qingquan, ZHAO Cunhu, HE Xiaoyong ,TIAN Xiaoyan, ZHANG Xiangqian, XI Xianmei （Plant Protection Institute,Inner Mongolia Academy of Agricuhural ＆ Animal Husbandry Sciences,Hohhot 010031, China）. Analysis of genetic diversity of the main agronomic traits of chickpea germplasm resources [J]. Journal of Northern Agriculture, 2018, 46(5): 9-18.