Abstract: 【Objective】To establish an efficient extraction method for solanine from potato peel,explore the application value and prospect of potato peel in plant-derived pesticides,and provide scientific evidence for the comprehensive utilization of solanine as a plant-derived pesticide.【Methods】Potato peel was used as the experimental material. Ultrasound-combined enzyme extraction method,acid enzyme solution extraction method,ultrasound-assisted extraction method,and acid water extraction method,four extraction methods for main component of solanine were compared and optimized. Single-factor experiments and the Box-Behnken experimental design from response surface analysis were used to optimize the extraction methods. The ultrasound-combined enzyme extraction method was optimized for solanine extraction.【Results】 Among the four extraction methods,the extraction efficiency from high to low was ultrasound-combined enzyme extraction method > acid enzyme solution extraction method > ultrasound-assisted extraction method > acid water extraction method. Among them,the content of solanine extract by ultrasound-combined enzyme extraction method was the highest,with α-chaconine at 8.949 mg/g DW and α-solanine at 8.058 mg/g DW,the extraction rate reached 0.850%. Based on the response surface analysis,the optimized regression equation model was established（Y_{Total content}=2.64-0.56×A-0.31×B+0.20×C+0.19×AB-0.11×AC+0.53×BC-0.74×A²-0.59×B²-0.50×C²）. With the maximum total content as the optimization objective,the optimal conditions were predicted to be enzyme amount at 1.30%,enzymatic hydrolysis time of 1.35 h,and ethanol volume fraction of 70.40%. Under these conditions,the actual total content of solanine extract was 3.149 mg/g DW,the difference with the predicted total content of 2.814 mg/g DW was within 5%,confirming a good correlation between the predicted value and experimental values.【Conclusion】The method of extracting solanine from potato peel by ultrasound-combined enzyme extraction method was established for the first time.The optimal extraction method obtained by response surface analysis optimization is reasonable,and the extraction efficiency of this method is higher,which can be used for industrial extraction of solanine.

Key words: Potato peel, Solanine, Ultrasound-combined enzyme extraction method, Response surface analysis