绿色荧光蛋白在植物与病原菌互作研究中的应用

doi:10.3969/j.issn.2096-1197.2019.06.12

Abstract

Abstract: As a reporter gene,green fluorescent protein （GFP） has the advantages of high sensitivity,good stability,low cytotoxicity and so on,which has been widely used in the study of the interaction between pathogens and host plants.In this paper,the application of GFP in plant pathogens was discussed from the aspects of transformation method of GFP,the pathogen infection process,the detection of pathogen quantity,and the study on subcellular structure of pathogen.It provided reference information for the application of GFP.

Key words: Green fluorescent protein, Pathogen, Reporter gene

CLC Number:

S432.1

SHI Zhidan, SONG Peiling, HAO Lifen, HUANGFU Haiyan, YAN Mengjiao, YANG Yongqing, GUO Chen, JIA Xiaoqing, HUANGFU Jiuru, LI Ziqin, ZHANG Baohui, CHEN Feifei. Application of green fluorescent protein in the study of the interaction between plant and pathogens[J].Journal of Northern Agriculture, 2019, 47(6): 68-72.

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https://journal30.magtechjournal.com/bfnyxb/EN/Y2019/V47/I6/68

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Application of green fluorescent protein in the study of the interaction between plant and pathogens

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