Abstract: The effect of Phytohormone 6-BA and KT on Ammpiptanthus Mongolicus shoot differentiation during tissue culture had been studied with stem apex, stem segment, cotyledon, hypocotyl as explants respectively. Results showed that： shoots can regenerate from stem apex and stem segment when culture in MS medium with 1 and 2mg/L 6-BA, but the shoot leaf then vitrification and died. Normal shoot can formed when cuhured in low 6-BA medium （0.5,0.1,Omgh~）. The shoot differentiation frequency is better higher in 0.5mg/L 6-BA. 0.5mg/L 6-BA can increase the differentiation frequency of cotyledon explants, and 0.5mg/L KT can increase the shoot differentiation frequency of hypocotyt during the differentiation of cotyledon and hypocotyl.