小天仙子组织培养研究

Abstract

Abstract: In order to protect wild resources and realize artificial cultivation, the tender stems of Hyoscyamus bohemicus were used as material to do the research on callus induction and differentiation, rooting and transplanting of tube seedlings, finally establish the clone of Hyoscyamus bohemicus. The results show that the optimum medium for callus induction and subculture is MS＋BA0.4mg/L＋2,4-D1.2mg/L＋NAAO.4mg/.L.The optimum medium for callus differentiation is MS＋6-BAI.Omg/L＋NAAO.lmg/ L.1/3MS＋NAA0.1mg/L＋IAAO.Smg/L is the optimum medium for rooting and subculture.Colonization of plantlets grows vigorously and it in maintained for all biological traits which wild plant has.

CLC Number:

S567.2

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Study on the Tissue Culture of Hyoscyamus Bohemicus Schmidt

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