披碱草属牧草DNA指纹图谱构建及遗传多样性分析

doi:10.3969/j.issn.2096-1197.2019.05.01

Abstract

Abstract: The study provides reference materials for the research,breeding and variety identification germplasm resources of Elymus.The DNA fingerprints of 26 Elymus germplasm materials （including 9 Registered varieties）were constructed using ISSR and SRAP molecular markers and the genetic diversity was analyzed.The results were as follows：95 bands were amplified clearly using 10 single ISSR primers.90 of 95 bands are polymorphic and the percentage of polymorphic bands is 94.74%.In addition,110 bands were amplified clearly using 10 pairs of SRAP primers.100 of the 110 amplified bands are polymorphic and the percentage of polymorphic bands is 90.91%.The genetic similarity coefficient（GS）among the 26 examined Elymus germplasm materials was 0.57~0.89,with an average of 0.73,indicating a rich genetic diversity.UPGMA cluster analysis was carried out based on the GS values and the 26 Elymus germplasm materials were divided into four groups at the GS value of 0.67.The DNA fingerprints of 26 Elymus germplasm materials were constructed using the ISSR primer UBC818 and the SRAP primers me4+em16.

Key words: Elymus, ISSR, SRAP, Genetic diversity, DNA fingerprinting

CLC Number:

S543.9

ZHANG Rui, TANG Aijia, XIE Jihong, SHI Fengling, ZHAO Yan. DNA fingerprinting and genetic diversity analysis of Elymus pastures[J].Journal of Northern Agriculture, 2019, 47(5): 1-8.

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References

[1] 孙建萍,袁庆华.披碱草属种质资源研究进展[J].草业科学,2005(12):2-5.
[2] GUSTAVO. DNA Markers[M].New York:Wiley-VCH,1998.
[3] 贾继增. 分子标记种质资源鉴定和分子标记育种[J].中国农业科学,1996,29(4):1-10.
[4] 魏臻武. 利用SSR、ISSR和RAPD技术构建苜蓿基因组DNA指纹图谱[J].草业学报,2004(3):62-67.
[5] ZIETKIEWICZ E,RAFALSKI A,LABUDA D .Genome fingerprinting by simple sequence repeat (SSR)-anchored polymerase chain reaction amplification[J]. Genomics,1994,20(2):176-183.
[6] GILBERT J E,LEWIS R V,WILKINSON M J,et al.Developing an appreciate strategy toassess genetic variability in plant germplasm collections[J]. Theoretical and Applied Genetics,1999,98(6/7):1125-1131.
[7] 钱韦,葛颂,洪德元.采用RAPD和ISSR标记探讨中国疣粒野生稻的遗传多样性[J].植物学报,2000,42(7):741-750.
[8] 马朝芝,傅廷栋,TUEVESSON S,等.用ISSR标记技术分析中国和瑞典甘蓝型油菜的遗传多样性[J]. 中国农业科学,2003,36(11):1403-1408.
[9] LI G,QUIROS C F.Sequence-related amplified polymo-rphism(SRAP),a new marker system based on a simple PCR reaction:its application to mapping and gene tagging in Brassica[J].Theoretical and Applied Genetics,2001,103(2/3):455-461.
[10] 林忠旭,张献龙,聂以春,等. 棉花SRAP遗传连锁图构建[J]. 科学通报,2003,48(15):1676-1679.
[11] FERRIOL M,PICÓ B,NUEZ F.Genetic diversity of a germplasm collection of cucurbita pepo using srap and aflp markers[J]. Theoretical and Applied Genetics,2003,107(2):271-282.
[12] 林忠旭,张献龙,聂以春.新型标记SRAP在棉花F₂分离群体及遗传多样性评价中的适用性分析[J].遗传学报,2004,31(6):622-626.
[13] LI G,GAO M,YANG B,et al.Gene for gene alignment between the Brassica and Arabidopsis genomes by direct transcriptomemapping[J]. Theoretical and Applied Genetics,2003,107(1):168-180.
[14] 郑经红,陈仕勇,陈智华,等.川西北高原野生垂穗披碱草遗传多样性的ISSR分析[J].西南民族大学学报(自然科学版),2014,40(3):330-335.
[15] ROHLF J F.NTSYS-PC:Numerical Taxonomy and Multivariate Analysis System[M].2nd ed.New York:Exeter Software,1994.
[16] 张连义,赵景娣,刘红葵,等.指纹图谱技术在牧草种和品种鉴定上的应用及其研究进展[J].畜牧与饲料科学,2007,28(1):41-43.
[17] 郭春苗,李宁,周晓明,等.基于SSR标记的葡萄品种(系)遗传多样性分析与指纹图谱构建[J].新疆农业科学,2015,52(11):2051-2058.
[18] 苗佳敏,张新全,陈智华,等.青藏高原和新疆地区垂穗披碱草种质的SRAP及RAPD分析[J].草地学报,2011,19(2):306-316.
[19] 李小雷,鲍红春,于卓.老芒麦和紫芒披碱草及其杂种F₁代与BC₁代的ISSR分析[J].种子,2016,35(12):17-20.
[20] 陈智华,苗佳敏,钟金城,等.野生垂穗披碱草种质遗传多样性的SRAP研究[J].草业学报,2009,18(5):192-200.

DNA fingerprinting and genetic diversity analysis of Elymus pastures

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