畜牧与饲料科学 ›› 2008, Vol. 29 ›› Issue (5): 9-9.doi: 10.12160/j.issn.1672-5190.2008.05.004

• 试验研究 • 上一篇    下一篇

稀释液中添加PCS对冷冻保存过程中山羊精子质量的影响

倪利平[1,3] 郑云胜[1] 宏伟[1] 田东海[2] 卢永跃[2] 邓常胜[2] 王利萍[2] 张小宇[2] 王建军[3] 陈福泉[3] 薛翠兰[3]   

  1. [1]内蒙古农业大学动物科学与医学学院,内蒙古呼和浩特010018 [2]内蒙古农业大学职业技术学院,内蒙古萨拉齐014109 [3]内蒙古巴彦淖尔市磴口县畜牧业局,内蒙古磴口015200
  • 出版日期:2008-10-20 发布日期:2008-10-20
  • 通讯作者: 倪利平
  • 作者简介:倪利平(1979-),男,在读硕士研究生,主要研究方向为动物繁殖技术。 通讯作者:郑云胜(1958-),男,教授,主要研究方向为动物繁殖技术,E-mail:yszheng@yahoo.com.cn。

Effect of PCS Addition to Extender on the Quality of Goat Spermatozoa during Freezing Process

NI Li-ping, ZHENG Yun-sheng, HONG Wei,TIAN Dong-hai, LU Yong-yue,DENG Chang-sheng, WANG Li-ping, ZHANG Xiao-yu, WANG Jian-jun, CHEN Fu-quan, XUE Cui-lan(1.College of Animal Science and Veterinary, hmer Mongolia Agricultural University, Huhhot 010018,China;2.Vocational and Technical College,Inner Mongolia Agricultural University,Salaqi 014109,China;3.Animal Husbandry Bureau of Deng Kou , dengkou 015200, China )   

  • Online:2008-10-20 Published:2008-10-20

摘要: 笔者以绒山羊为试验动物,在卵黄稀释液中添加胆固醇硫酸脂(PCS),研究了在冷冻过程中PCS对精子质量的影响。添加PCS共设计了0.15,0.10,0.50g/100mL的添加量,总体分析以0.10g/100mL添加量为最佳,EY试验组显著好于EY对照组。其中,EY组解冻后精子活率、膜完整率、项体完整率分别是34.94%、39.17%、75.24%;EY对照组分别是28.74%、30.70%、64.70%。

Abstract: This paper studied on the effect of potassium cholesterol sulfate (PCS) to the spermatozoa quality during freezing process. The test group of addition of PCS to EY dilution consisted of three group. The addition was 0.15 g/100 mL, 0.10 g/100 mL and 0.5 g/100 mL respectively. The result indicated: the optimal addition was 0.10 g/100 mL.The test group' sperm quality of EY diluent is better than the control group of EY diluent. After being thawing, the motility, plasma membrane integrity and atropine integrity of the EY group were 34.94 %, 39.17 %, 75.24 %, but the EY control group were 28.74 %, 30.70 %, 64.70 %.

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