Abstract: The regeneration technology of 2 tall fescue varieties （Xinxiu and Yemingzhu No. 2） was studied, the high frequency regeneration system was set up and foundation for genetic transformation was laid. With mature seeds as explant, embryogenic callus was induced. The best culture medium for embryogenic callus induction of 2 varieties was MS＋2, 4-D 5.0 mg/L＋6-BA 0.1 mg/L. The optimal medium for callus differentiation was MS＋6-BA 2.0 mg/L for Xinxiu and MS＋6-BA 1.0 mg/L for Yemingzhu No. 2, and the optimum medium for rooting was MS＋NAA 0.5 mg/L.