畜牧与饲料科学 ›› 2009, Vol. 30 ›› Issue (4): 8-8.doi: 10.12160/j.issn.1672-5190.2009.04.004

• 基础科学 • 上一篇    下一篇

烟草BY2细胞培养及盐胁迫研究

吴科瀛 梁玉玲   

  1. 河北大学生命科学学院,河北保定071002
  • 出版日期:2009-04-20 发布日期:2009-04-20
  • 通讯作者: 吴科瀛
  • 作者简介:吴科瀛(1982-),女,硕士,主要研究方向为生物化学与分子生物学。 通讯作者:梁玉玲(1964-),女,教授,主要研究方向为植物基因工程与分子生物学,E-mail:yuling_liang@163.com。

Study on Tobacco BY2 Cell Culture and Salt Stress

WU Ke-ying, LIANG Yu-ling (College of Life Science, Hebei University, Baoding 071002, China)   

  • Online:2009-04-20 Published:2009-04-20

摘要: 对比了2种不同配方的培养基对烟草BY2细胞生长的影响,试验表明,2种培养基对于继代培养BY2细胞并无过多差异。将烟草BY2细胞进行盐胁迫处理,并检测细胞增重等生理生化指标,结果显示,当盐浓度达到0.5%时,BY2细胞已经受到严重损伤。

Abstract: Bright Yellow 2 cell is a very useful cell line in various study fields. Two different formula of BY2 culture medium were compared in this study to explore the influence to BY2 cell. The results showed that there is no big difference between the two medium. Salt stress results showed that BY2 cell was sever damaged under 0.5 % NaCl stress.

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