Abstract: The objective of this study was to characterize the antimicrobial resistance profile of fecal Escherichia coli from calves of 30 days of age in a cattle farm in Xinjiang. Fecal samples were collected from 92 30-day-old calves; the Escherichia coli strains were isolated and identified with conventional methods; antimicrobial sensitivity of the isolates was assessed by disk diffusion method （K-B method）. The results showed that a total of 92 Escherichia coli strains were obtained from the 92 fecal samples, indicating the isolating rate was 100%; high resistance rate were observed against amikacin （93.75%）, enrofloxacin （64.50%）, norfloxacin （59.30%） and gentamicin （57.14%）, and the resistance rate were all above 50%; isolates also exhibited certain resistance to amoxicillin/clavulanic acid potassium （36.30%）, amoxicillin （26.00%） and ciprofloxacin （13.60%）; the highest susceptibility were observed in ampicillin and only 3.03% of the isolates were resistant to this antimicrobial agent. These results indicate that the fecal Escherichia coli strains from caves in this cattle farm were resistant to some commonly used antimicrobial agents, and we suggest that the employment of antibiotics for clinical treatment should be guided by the results of antimicrobial susceptibility test.