滩羊羔羊瘤胃和小肠菌群多样性的比较研究

doi:10.12160/j.issn.1672-5190.2022.04.008

畜牧与饲料科学 ›› 2022, Vol. 43 ›› Issue (4): 48-56.doi: 10.12160/j.issn.1672-5190.2022.04.008

滩羊羔羊瘤胃和小肠菌群多样性的比较研究

金鹿¹,桑丹¹,张崇志¹,李胜利¹,张春华¹,张俊丽²,施安²,孙海洲¹,李聚才²

1.内蒙古自治区农牧业科学院动物营养与饲料研究所/内蒙古自治区草食动物营养科学重点实验室，内蒙古呼和浩特 010031
2.宁夏农林科学院动物科学研究所，宁夏银川 750002

收稿日期:2022-02-28 出版日期:2022-07-30 发布日期:2022-07-21
通讯作者: 孙海洲（1970—），男，研究员,博士,主要研究方向为动物营养与饲料科学。李聚才（1960—），男，研究员，主要研究方向为动物营养与饲料科学。
作者简介:金鹿（1987—），女，副研究员，博士，主要研究方向为动物营养与饲料科学。|桑丹（1978—），女，助理研究员，博士，主要研究方向为动物营养与饲料科学。
基金资助:
国家绒毛用羊产业技术体系(CARS-39-11);宁夏对外科技合作专项(DW-X-2018017);内蒙古自治区“草原英才”创新人才团队项目;“草原英才”工程培养（引进）高层次人才项目;内蒙古自治区草食动物营养科学重点实验室(2022CXJJM14)

Comparison of Microbial Diversity in Rumen and Small Intestine of Tan Sheep Lambs

JIN Lu¹,Sangdan ¹,ZHANG Chong-zhi¹,LI Sheng-li¹,ZHANG Chun-hua¹,ZHANG Jun-li²,SHI An²,SUN Hai-zhou¹,LI Ju-cai²

1. Animal Nutrition and Feed Research Institution，Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences/Inner Mongolia Key Laboratory of Herbivores Nutrition，Hohhot 010031，China
2. Animal Sciences Institute，Ningxia Academy of Agricultural and Forestry Sciences，Yinchuan 750002，China

Received:2022-02-28 Online:2022-07-30 Published:2022-07-21

摘要/Abstract

摘要：

[目的]研究滩羊羔羊瘤胃和小肠菌群多样性的差异。[方法]选取健康的断奶滩羊公羔，屠宰后采集瘤胃、十二指肠、空肠和回肠内容物，利用16S rDNA高通量测序技术分析瘤胃和小肠菌群结构及多样性。[结果]十二指肠样品Chao1指数高于瘤胃、空肠和回肠，瘤胃样品Shannon指数和Simpson指数均高于其他部位，但差异不显著（P>0.05）。瘤胃液中拟杆菌门（Bacteroidetes）、螺旋菌门（Spirochaetae）、丝状杆菌门（Fibrobacteres）的相对丰度显著（P<0.05）高于十二指肠、空肠和回肠，十二指肠中广古菌门（Euryarchaeota）的相对丰度与瘤胃相比有升高的趋势（0.05<P<0.10），空肠和回肠中厚壁菌门（Firmicutes）的相对丰度显著（P<0.05）高于十二指肠。瘤胃中的未鉴定菌属（Unidentified）、理研菌属_RC9（Rikenellaceae_RC9_gut_group）、密螺旋体属_2（Treponema_2）、瘤胃球菌属_NK4A214（Ruminococcaceae_NK4A214_group）、普雷沃菌属_UCG-001（Prevotellaceae_UCG-001）相对丰度显著（P<0.05）高于十二指肠、空肠和回肠，空肠中Family_ⅩⅢ_AD3011_group相对丰度显著（P<0.05）高于其他部位。[结论]滩羊羔羊瘤胃中细菌的多样性更丰富，瘤胃微生物与十二指肠、空肠和回肠的细菌区系显著不同。

关键词: 滩羊, 瘤胃, 小肠, 菌群多样性

Abstract:

[Objective] To elucidate the differences in microbial diversity between rumen and small intestine of Tan sheep lambs. [Method] Healthy weaned Tan sheep male lambs were selected and slaughtered, and the ruminal, duodenal, jejunal and ileac contents were sampled. The microbial composition and diversity of rumen and small intestine were assessed using 16S rDNA high-throughput sequencing. [Result] The Chao1 index of duodenal samples was higher than that of ruminal, jejunal and ileac samples, and the Shannon index and Simpson index of ruminal samples were higher than those of the other parts, although the difference was not statistically significant （P>0.05）. The relative abundance of Bacteroidetes, Spirochaetae and Fibrobacteres in ruminal fluid was significantly （P<0.05） higher than that in duodenum, jejunum and ileum. The relative abundance of Euryarchaeota in duodenum had an increasing trend compared with that in rumen（0.05<P<0.10）. The relative abundance of Firmicutes in jejunum and ileum was significantly （P<0.05） higher than that in duodenum. In rumen, the relative abundance of unidentified bacteria group, Rikenellaceae_RC9_gut_group, Treponema_2, Ruminococcaceae_NK4A214_group and Prevotellaceae_UCG-001 was significantly （P<0.05） higher than that in duodenum, jejunum and ileum. The relative abundance of Family_ⅩⅢ_AD3011_group in jejunum was significantly （P<0.05） higher than that of the other parts. [Conclusion] The rumen of Tan sheep lambs contained a richer diversity of bacteria, and these bacteria differed greatly from those found in duodenum, jejunum and ileum.

Key words: Tan sheep, rumen, small intestine, microbial diversity

中图分类号:

S826.1

金鹿, 桑丹, 张崇志, 李胜利, 张春华, 张俊丽, 施安, 孙海洲, 李聚才. 滩羊羔羊瘤胃和小肠菌群多样性的比较研究[J]. 畜牧与饲料科学, 2022, 43(4): 48-56.

JIN Lu, Sangdan , ZHANG Chong-zhi, LI Sheng-li, ZHANG Chun-hua, ZHANG Jun-li, SHI An, SUN Hai-zhou, LI Ju-cai. Comparison of Microbial Diversity in Rumen and Small Intestine of Tan Sheep Lambs[J]. Animal Husbandry and Feed Science, 2022, 43(4): 48-56.

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参考文献 37

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滩羊羔羊瘤胃和小肠菌群多样性的比较研究

Comparison of Microbial Diversity in Rumen and Small Intestine of Tan Sheep Lambs

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