睾酮对新生期大鼠支持细胞和精原细胞Adamts16表达的影响

doi:10.12160/j.issn.1672-5190.2023.05.003

畜牧与饲料科学 ›› 2023, Vol. 44 ›› Issue (5): 15-21.doi: 10.12160/j.issn.1672-5190.2023.05.003

睾酮对新生期大鼠支持细胞和精原细胞Adamts16表达的影响

李倩倩¹,王东阳²,白萨日娜¹,刘建琦¹,包图雅¹,郭晓珍¹

1.内蒙古医科大学基础医学院，内蒙古呼和浩特 010110
2.赤峰学院附属医院生殖医学科，内蒙古赤峰 024000

收稿日期:2023-09-08 出版日期:2023-09-30 发布日期:2023-11-14
通讯作者: 包图雅（1978—），女，副教授，博士，主要研究方向为组织胚胎学、生殖医学与生殖健康。郭晓珍（1989—），女，助教，硕士，主要研究方向为病理生理学、组织胚胎学。
作者简介:李倩倩（1992—），女，硕士研究生，主要研究方向为生殖医学与生殖健康。
基金资助:
内蒙古自然科学基金项目(2019MS03008);2020年度内蒙古自治区留学人员创新创业支持启动计划;内蒙古自治区卫生健康委员会2022年度自治区卫生健康科技计划项目(202201194);内蒙古医科大学面上项目(YKD2023MS032)

Effects of Testosterone on the Expression of Adamts16 Gene in Sertoli Cells and Spermatogonia of Neonatal Rats

LI Qianqian¹,WANG Dongyang²,BAI Sarina¹,LIU Jianqi¹,BAO Tuya¹,GUO Xiaozhen¹

1. School of Basic Medical Sciences，Inner Mongolia Medical University，Hohhot 010110，China
2. Department of Reproductive Medicine，Affiliated Hospital of Chifeng University，Chifeng 024000，China

Received:2023-09-08 Online:2023-09-30 Published:2023-11-14

摘要/Abstract

摘要：

[目的]研究睾酮对新生期大鼠支持细胞和精原细胞中血小板反应蛋白解整合素金属肽酶16（a disintegrin and metalloprotease with thrombospondin motifs 16，Adamts16）基因表达的影响，以及Adamts16基因与大鼠隐睾的关系。[方法]利用RT-qPCR技术检测不同浓度睾酮（10^-6、10^-7、10^-8、10^-9 mol/L）培养大鼠支持细胞和精原细胞6、12、24 h后Adamts16 基因的mRNA相对表达量，确定睾酮诱导支持细胞和精原细胞Adamts16基因mRNA表达的最佳剂量和最佳时间。应用HE染色法观察出生2、4、8 d野生型和Adamts16基因敲除型大鼠的睾丸位置。[结果]用10^-6 mol/L睾酮诱导大鼠支持细胞6 h 时Adamts16基因的mRNA相对表达量最高。用10^-7 mol/L睾酮诱导大鼠精原细胞24 h时Adamts16基因mRNA相对表达量最高。与野生型大鼠相比，Adamts16基因敲除大鼠睾丸下降速度减缓。[结论]睾酮可促进新生期大鼠睾丸支持细胞和精原细胞Adamts16基因的mRNA表达；Adamts16基因缺失会引起大鼠隐睾症。

关键词: 睾酮, 支持细胞, 精原细胞, Adamts16基因

Abstract:

[Objective] This study was conducted to investigate the effects of testosterone on the expression of a disintegrin and metalloprotease with thrombospondin motifs 16 （Adamts16） gene in sertoli cells and spermatogonia of neonatal rats, and to assess the relationship between the Adamts16 gene and rat cryptorchidism. [Method] RT-qPCR assay was used to detect the relative mRNA expression levels of Adamts16 gene in sertoli cells and spermatogonia of rats cultured with different concentrations of testosterone （10^-6, 10^-7, 10^-8 and 10^-9 mol/L） for 6, 12 and 24 h, respectively. The optimal concentration and time for testosterone to induce the expression of Adamts16 gene in mRNA level in sertoli cells and spermatogonia was determined. The testicular position of wild type and Adamts16 gene knockout rats 2, 4 and 8 days after birth was observed using HE staining. [Result] The highest relative mRNA expression level of Adamts16 gene in sertoli cells of rats was observed when induced with 10^-6 mol/L testosterone for 6 h, and that in spermatogonia was found when induced with 10^-7 mol/L testosterone for 24 h. Compared with the wild type rats, Adamts16 gene knockout rats had slower orchiocatabasis. [Conclusion] Testosterone could promote the mRNA expression of Adamts16 gene in sertoli cells and spermatogonia of neonatal rats. The deletion of Adamts16 gene could cause cryptorchidism in rats.

Key words: testosterone, sertoli cell, spermatogonia, Adamts16 gene

中图分类号:

李倩倩, 王东阳, 白萨日娜, 刘建琦, 包图雅, 郭晓珍. 睾酮对新生期大鼠支持细胞和精原细胞Adamts16表达的影响[J]. 畜牧与饲料科学, 2023, 44(5): 15-21.

LI Qianqian, WANG Dongyang, BAI Sarina, LIU Jianqi, BAO Tuya, GUO Xiaozhen. Effects of Testosterone on the Expression of Adamts16 Gene in Sertoli Cells and Spermatogonia of Neonatal Rats[J]. Animal Husbandry and Feed Science, 2023, 44(5): 15-21.

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睾酮对新生期大鼠支持细胞和精原细胞Adamts16表达的影响

Effects of Testosterone on the Expression of Adamts16 Gene in Sertoli Cells and Spermatogonia of Neonatal Rats

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