Abstract: The pathogenetic milk samples from a cattle-raising community in Tumoteyou County of Baotou were selected to perform tests for pathogen isolation and culturing,smear staining,biochemical identification,drug sensitivity to find out which type of mastitis pathogenic bacteria the cows had and which kind of drugs they were sensitive to. The results showed that the prevalence of cow mastitis in the community is caused by Escherichia coli,Streptococcus agalactiae and Staphylococcus aureus. The identified pathogens had certain drug-resistance to long-term soil enzyme and the sulfate chains of enzyme, but they were extremely sensitive to cephalexin and were also highly sensitive to the new enzyme and ampicillin sodium.