畜牧与饲料科学 ›› 2016, Vol. 37 ›› Issue (10): 22-22.doi: 10.12160/j.issn.1672-5190.2016.10.008

• 基础科学 • 上一篇    下一篇

不同稀释液对藏猪精液4℃保存效果的影响

陈晓英[1];马金英[1];宋天增[1];唐建华[2];赵彦玲[3];任子利[3];朱彦宾[1];王良润[4]   

  1. [1]西藏自治区农牧科学院畜牧兽医研究所,西藏拉萨850009 [2]西藏自治区畜牧总站,西藏托萨850000 [3]西藏农牧学院动物科学学院,西藏林芝860000 [4]西藏牧乐农业科技有限公司,西藏拉萨850009
  • 出版日期:2016-10-20 发布日期:2016-10-20
  • 通讯作者: 陈晓英
  • 作者简介:陈晓英(1981-),女,助理研究员,硕士,主要从事高原畜禽繁殖技术研究工作。 通讯作者:宋天增(1980-),男,助理研究员,硕士,主要研究方向为动物繁殖营养调控与遗传育种。
  • 基金资助:
    西藏自治区2016年高校青年教师创新支持计划项目(QCZ2016-47); 院(校)企合作技术开发项目(YXQ2015ZZ-01); 西藏自治区2016年农林业科技成果转化与推广(农牧业技术推广)项目“特色畜禽(藏猪)营养调控研究”

Preservation Effect of Different Extenders on Tibetan Boar Semen at 4℃

CHEN Xiao-ying1, MA Jin-ying1, SONG Tian-zeng1, TANG Jian-hua2, ZHAO Yan-ling3, REN Zi-li3, ZHU Yan-bin1, WANG Liang-run4 (1.Institute of Animal Science and Veterinary Medicine,Tibet Academy of Agricultural and Animal Husbandry Sciences,l,hasa 850009,China;2. Tibet Animal Husbandry Service Station,Lhasa 850000,China;3.Facuhy of Animal Science,Tibet Agricultural and Animal Husbandry College, Nyingchi 860000,China;4. Tibet Mulo Agricultural Sciences and Technology Co., Ltd.,Lhasa 850009,China)   

  • Online:2016-10-20 Published:2016-10-20

摘要: 为探讨不同精液稀释液对4℃保存藏猪精液的效果,保证优良藏猪种公猪精液的合理、高效利用,该试验选用5种常用的猪精液常温保存稀释液,并在藏猪精液4℃保存至第5天时检查藏猪精子活力、精子畸形率、精子顶体完整率、精子质膜完整率等。结果表明,在精液保存过程中,2号液对藏猪精液的保存效果最佳,精子活力在保存至第5天时仍然保持在0.54,且藏猪精子活力、精子畸形率、精子顶体完整率、精子质膜完整率均高于其余4组(P〈0.05)。该试验研究初步筛选了4℃保存藏猪精液的稀释液,为今后藏猪精液的生产应用奠定了基础。

Abstract: This study was conducted to investigate the preservation effect of different extenders on Tibetan Boar semen at 4 ℃ and to provide scientific basis for reasonable and high-efficient utilization of excellent Tibetan Boar breeder semen.Five different extenders commonly used for preservation of Tibetan Boar semen in room temperature were selected.The sperm motility,sperm deformity rate,and the integrity rate of sperm acrosome and plasma membrane preserved by the five extenders at the 5thday of preservation period at 4 ℃ were statistically evaluated and compared.The results revealed that the NO.2 extenders exhibited the best preservation effect with the sperm motility of 0.54 at the 5thday of preservation period,and the sperm motility,sperm deformity rate and the integrity rate of sperm acrosome and plasma membrane preserved with NO.2 extenders were higher than those of the semen preserved with the other four extenders(P〈0.05).This study preliminary selected the appropriate extenders for Tibetan Boar semen used at 4 ℃ and laid the foundation for the production and application of Tibetan Boar semen in the future.

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