畜牧与饲料科学 ›› 2016, Vol. 37 ›› Issue (3): 18-18.doi: 10.12160/j.issn.1672-5190.2016.03.006

• 基础科学 • 上一篇    下一篇

激动素对衰老模型小鼠免疫力的影响研究

武莹莹;李梦云;宋格格;闫小兰;张克喆;王刚   

  1. 河南科技大学,河南洛阳471023
  • 出版日期:2016-03-20 发布日期:2016-03-20
  • 通讯作者: 武莹莹
  • 作者简介:武莹莹(1997-),女,所学专业为动物医学, 通讯作者:李梦云(1974-),女,讲师,博士,主要研究方向为动物机能与形态、免疽及其生理调控.
  • 基金资助:
    河南科技大学大学生科研训练计划项目(SRTP项目,2015144);河南科技大学实验技术开发基金项目(SY1516040);河南科技大学教育教学改革项目(2015YB-017); 博士启动基金(4025/13480072)

Effect of Kinetin on Immunity of Aging Model Mice

WU Ying-ying, LI Meng-yun, SONG Ge-ge, YAN Xiao-lan, ZHANG Ke-zhe, WANG Gang (Henan University of Science and Technology, Luoyang 471023, China)   

  • Online:2016-03-20 Published:2016-03-20

摘要: 为研究激动素(Kn)对衰老模型小鼠免疫力的影响,将50只昆明系小白鼠随机分为衰老模型对照组、激动素处理组(低、中、高剂量组)、青年对照组,每组10只,雌雄各半。衰老模型对照组、激动素处理组小鼠背部皮下连续45 d注射D-半乳糖[125 mg/(kg·BW)]制备衰老模型,1次/d。试验第11天时,低、中、高剂量激动素处理组小鼠分别腹腔注射5、10、20 mg/(kg·BW)的激动素溶液,1次/d,直至第45天;同时期内衰老模型对照组和青年对照组小鼠颈背部皮肤注射生理盐水。试验结束时,计算各组小鼠脾指数,检测脾脏SOD、CAT、GSH-PX活性及MDA含量,检测血清IL-2、IL-6以及IgA、IgG、IgM含量。结果表明,经激动素处理后,小鼠的脾指数显著或极显著高于衰老模型对照组(P〈0.05或P〈0.01);3种抗氧化酶活性与衰老模型对照组相比有所提高,其中高剂量组的提高作用最为显著(P〈0.01);代谢物MDA含量显著或极显著低于衰老模型对照组(P〈0.05或P〈0.01);血清IL-2含量显著或极显著高于衰老模型对照组(P〈0.05或P〈0.01),血清IL-6含量显著或极显著低于衰老模型对照组(P〈0.05或P〈0.01),3种血清球蛋白含量与衰老模型对照组相比均有所提高,其中高剂量组的提高作用最为显著(P〈0.01)。综上提示,激动素具有显著的延缓衰老、抗氧化损伤、提高免疫力的作用。

Abstract: In order to investigate the effect of kinetin on immunity of aging model mice, a total of 50 mice of Kunming strain were selected and divided into aging model control group, low dosage kinetin group, moderate dosage kinetin group, high dosage kinetin group, and young control group. There were 10 mice in each group with half males and half females. The mice in aging model control group and kinetin treatment groups were subcutaneously administrated 125 mg/(kg·BW)of D-galactose once daily on the back for consecutive 45 d. On day 11, the mice in low, moderate and high dosage kinetin group were intraperitoneally administrated with 5, 10, 20 mg/(kg·BW)of kinetin solutions once daily till 45th day; in the same period, the mice in aging model control group and young control group were subcutaneously administrated with saline on the back of the neck. At the end of the experiment, the spleen index of the mice in different groups was calculated, the activity of SOD, CAT, GSH-PX and the content of MDA in spleen were determined, and the serum content of IL-2, IL-6, IgA, IgG, IgM were evaluated. The results revealed that the spleen index of kinetin treatment groups was significantly or extremely significantly higher than that of aging model control group(P0.05 or P0.01); the activity of the 3 tested antioxidant enzymes of kinetin treatment groups was increased in different degree compared with that of aging model control group, and the high dosage kinetin group exhibited extreme significance(P0.01); the content of MDA in spleen of kinetin treatment groups was significantly or extremely significantly reduced compared with that of aging model control group(P 0.05 or P 0.01); the serum content of IL-2 of kinetin treatment groups was significantly or extremely significantly higher than that of aging model control group(P 0.05 or P 0.01), and the serum content of IL-6 was significantly or extremely significantly lower than that of aging model control group(P0.05 or P0.01); the serum content of the 3 tested globulins of kinetin treatment groups was increased in different degree compared with that of aging model control group,and the high dosage kinetin group exhibited extreme significance(P0.01). Based on the results obtained in this study, it can be concluded that kinetin are capable to delay aging, resist oxidation damage and enhance immunity.

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