畜牧与饲料科学 ›› 2022, Vol. 43 ›› Issue (2): 84-89.doi: 10.12160/j.issn.1672-5190.2022.02.014

• 草业科学 • 上一篇    下一篇

不同氮肥施用量对阴山北麓饲用燕麦抗氧化酶活性的影响

田永雷1, 白春利1, 慕宗杰2, 孙林1, 娜娜1   

  1. 1.内蒙古自治区农牧业科学院草原研究所,内蒙古 呼和浩特 010031;
    2.内蒙古自治区农牧业科学院农牧业经济与信息研究所,内蒙古 呼和浩特 010031
  • 收稿日期:2022-02-08 出版日期:2022-03-30 发布日期:2022-03-30
  • 通讯作者: 白春利(1980—),女,研究员,博士,主要从事牧草栽培研究工作。
  • 作者简介:田永雷(1994—),男,研究实习员,博士研究生,主要从事牧草栽培研究工作。
  • 基金资助:
    内蒙古农牧业创新基金项目(2020CXJJM10); 内蒙古农牧业青年创新基金项目(2021QNJJM05); “科技兴蒙”行动重点专项巴彦淖尔国家农业高新技术产业示范区重点项目“区域性多羔肉羊选育及饲草料高效优化利用技术研究与示范”(NMKJXM202110)。

Effects of Different Nitrogen Fertilizer Application Rates on Activities of Antioxidant Enzymes in Feed Oat(Avena sativa)at the Northern Foot of Yinshan Mountain

TIAN Yong-lei1, BAI Chun-li1, MU Zong-jie2, SUN Lin1, Nana1   

  1. 1. Grassland Research Institute,Academy of Agricultural and Animal Husbandry Sciences,Hohhot 010031,China;
    2. Institute of Agricultural and Animal Husbandry Economy and Information,Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences,Hohhot 010031,China
  • Received:2022-02-08 Online:2022-03-30 Published:2022-03-30

摘要: [目的] 探讨内蒙古阴山北麓饲用燕麦抗氧化酶活性对氮肥的生理响应及差异变化,研究适宜的施氮量。[方法] 以饲用燕麦(Avena sativa)“三星”为材料,设置0、150、200、250、300、350 kg/hm2施氮水平,在苗期、分蘖期、拔节期、孕穗期分别追施氮肥用量的15%、35%、30%和20%,于孕穗期、抽穗期、灌浆期测定分析饲用燕麦叶片中3种抗氧化酶活性及丙二醛(MDA)含量。[结果] 随着施氮量的增加,孕穗期、抽穗期和灌浆期饲用燕麦叶片的超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)活性呈现先增加后降低的趋势,MDA的含量逐渐降低。3个时期的饲用燕麦叶片POD活性均在施氮水平为250 kg/hm2时最高;孕穗期和灌浆期的饲用燕麦叶片SOD活性在施氮水平为250 kg/hm2时最高;孕穗期和抽穗期饲用燕麦叶片CAT活性在施氮水平为300 kg/hm2时最高,灌浆期CAT活性在施氮水平为250 kg/hm2时最高。[结论] 内蒙古阴山北麓施氮肥对饲用燕麦抗氧化酶活性的影响为低氮促进、高氮抑制,适宜施氮量为250 kg/hm2

关键词: 阴山北麓, 饲用燕麦, 施氮量, 抗氧化酶

Abstract: [Objective] To assess the physiological response and differential changes of the activities of antioxidant enzymes in feed oat (Avena sativa) to nitrogen fertilizer at the northern foot of Yinshan Mountain, and to determine the optimal nitrogen fertilizer application rate. [Method] Using feed oat species ′Sanxing′ as the material, the nitrogen fertilizer application rates of 0, 150, 200, 250, 300 and 350 kg/hm2 were set, and additional 15%, 35%, 30%, and 20% of the nitrogen fertilizer was applied at seedling stage, tillering stage, jointing stage, and booting stage for each nitrogen fertilizer application rate, respectively. The activities of three types of antioxidant enzymes and the level of malondialdehyde (MDA) in feed oat leaves were evaluated at booting stage, heading stage and grain filling stage. [Result] As the increase of nitrogen fertilizer application rates, the activities of superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) in feed oat leaves at booting stage, heading stage, and grain filling stage increased first and then declined, and the level of MDA reduced progressively. At all of the three observed growth stages, the highest POD activity in feed oat leaves was found at the nitrogen fertilizer application rate of 250 kg/hm2. At booting stage and grain filling stage, feed oat leaves had the highest SOD activity at the nitrogen fertilizer application rate of 250 kg/hm2. The highest CAT activity was observed at the nitrogen fertilizer application rate of 300 kg/hm2 at booting stage and heading stage, and at grain filling stage, that was observed at the nitrogen fertilizer application rate of 250 kg/hm2. [Conclusion] At the northern foot of Yinshan Mountain, lower application rate of nitrogen fertilizer enhances the activities of antioxidant enzymes in feed oat, and higher rate exerts the reverse effect. The appropriate nitrogen fertilizer application rate is 250 kg/hm2.

Key words: the northern foot of Yinshan Mountain, feed oat, nitrogen fertilizer application rate, antioxidant enzyme

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