畜牧与饲料科学 ›› 2023, Vol. 44 ›› Issue (6): 22-30.doi: 10.12160/j.issn.1672-5190.2023.06.003

• 动物营养与饲料科学 • 上一篇    下一篇

棉酚对雄性肉仔鸡生长性能、血清抗氧化能力及肾脏病理组织形态的影响

李啊诗, 刘盼, 张新楠, 胡雨露, 李玉杰, 赵扬, 吴尚泽, 贺建忠, 张梦迪   

  1. 塔里木大学动物科学与技术学院,新疆 阿拉尔 843300
  • 收稿日期:2023-09-01 出版日期:2023-11-30 发布日期:2024-01-11
  • 通讯作者: 张梦迪(1996—),女,讲师,硕士,主要从事动物营养代谢病研究工作。
  • 作者简介:李啊诗(2000—),女,所学专业为动物医学。
  • 基金资助:
    2022年度塔里木大学校长基金硕士人才项目(TDZKSS202238)(2022ZD160); 2023年塔里木大学大学生创新创业训练计划项目(202310757003); 2022年塔里木大学大学生创新创业训练计划项目(202210757002)

Effects of Gossypol on Growth Performance,Serum Antioxidant Capacity and Renal Pathological Morphology in Male Broilers

LI Ashi, LIU Pan, ZHANG Xinnan, HU Yulu, LI Yujie, ZHAO Yang, WU Shangze, HE Jianzhong, ZHANG Mengdi   

  1. College of Animal Science and Technology,Tarim University,Alar 843300,China
  • Received:2023-09-01 Online:2023-11-30 Published:2024-01-11

摘要: [目的]研究棉酚对不同生长阶段雄性肉仔鸡生长性能、血清抗氧化能力及肾脏病理组织形态的影响,为肉鸡养殖中棉籽粕的合理应用积累基础试验数据。[方法]选取48只同批次1日龄健康雄性良凤花肉仔鸡,根据体重相近原则分为4组:对照组(C)、低剂量组(L)、中剂量组(M)和高剂量组(H),每组4个重复,每个重复3只。在饲喂基础日粮基础上,C组以及L组、M组、H组每天分别灌胃蒸馏水以及100、150、200 mg/(kg·BW)棉酚1次,直至20日龄,共灌胃20 d;试验期间,记录各组肉仔鸡的采食及增重情况,计算不同生长阶段的料重比;分别于6、11、16和21日龄采集肉仔鸡血液样本,测定血清过氧化氢酶(catalase,CAT)和谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)活力;于21日龄采集肾脏组织样本,制备HE染色切片,观察肾脏病理组织学变化。[结果]1~15日龄阶段,L组、M组和H组的料重比极显著(P<0.01)高于C组;6~15日龄阶段,M组的料重比极显著(P<0.01)高于L组;16~20日龄阶段,L组和H组的料重比极显著(P<0.01)高于C组,M组的料重比呈负增长。6~21日龄阶段,C组的血清CAT和GSH-Px活力呈上升趋势,L组、M组和H组均呈下降趋势;11日龄时,L组的血清CAT和GSH-Px活力显著(P<0.05)低于C组,M组和H组的血清GSH-Px活力极显著(P<0.01)低于C组;16日龄和21日龄时,L组、M组和H组的血清CAT和GSH-Px活力均极显著(P<0.01)低于C组;21日龄时,M组的血清GSH-Px活力极显著(P<0.01)低于L组。病理组织学观察显示,棉酚灌胃组肉仔鸡的肾小球肿胀,肾小囊间隙变窄,肾小管坏死,肾组织受损伤程度为M组>H组>L组。[结论]从1日龄开始给雄性肉仔鸡连续灌喂20 d剂量为100、150和200 mg/(kg·BW)的棉酚,可降低其生长性能及血清抗氧化能力,并对肾脏造成损伤;随着灌胃时间的延长,以150 mg/(kg·BW)的剂量影响最为明显。

关键词: 棉酚, 雄性肉仔鸡, 生长性能, 血清抗氧化酶, 肾脏病变

Abstract: [Objective] This study aimed to assess the effects of gossypol on growth performance, serum antioxidant capacity, and renal pathological morphology of male broilers at different growth stages, so as to accumulate basic experimental data for the rational application of cotton seed meal in broiler breeding. [Method] A total of 48 healthy 1-day-old Liangfenghua male broilers from the same batch were selected and randomly allocated to 4 groups according to the principle of similar body weight, including a control group (C), a low-dose group (L), a medium-dose group (M), and a high-dose group (H). Each group had 4 replicates, with 3 broilers per replicate. The experimental broilers in different groups fed the same basal diet. In addition, the broilers in L, M and H group were orally administered 100, 150 and 200 mg/(kg·BW) gossypol once a day until 20-day-old, for a total of 20 days, respectively, while those in C group were orally given distilled water. During the experiment, the feed intake and weight gain of the broilers in each group were recorded, and the feed to weight gain ratio at different growth stages were calculated. Blood samples were collected at 6, 11, 16 and 21 days of age, and the serum activities of catalase (CAT) and glutathione peroxidase (GSH-Px) were measured. Kidney tissue samples were collected at 21 days of age, with HE staining sections prepared, and changes in renal histopathology observed. [Result] At 1-15 days of age, the feed to weight gain ratios of L, M and H groups were extremely significantly (P<0.01) higher than that of C group; at 6-15 days of age, the feed to weight gain ratio of M group was extremely significantly (P<0.01) higher than that of L group; at 16-20 days of age, the feed to weight gain ratios of L and H groups were extremely significantly (P<0.01) higher than that of C group, while that of M group had negative increase. At 6-21 days of age, the serum activities of CAT and GSH-Px in C group showed upward trends, while those in L, M and H groups all showed downward trends; at 11 days of age, the serum activities of CAT and GSH-Px in L group were significantly (P<0.05) lower than those in C group, while the serum activity of GSH-Px in M and H groups was extremely significantly (P<0.01) lower than that in C group; at 16 and 21 days of age, the serum activities of CAT and GSH-Px in L, M and H groups were all extremely significantly (P<0.01) lower than those in C group; at 21 days of age, the serum activity of GSH-Px in M group was extremely significantly (P<0.01) lower than that in L group. Pathological histology observations showed that the renal glomeruli of the broilers given gossypol by gavage were swollen, the renal capsule space became narrower, and the renal tubules were necrotic.The degree of renal tissue damage was M group>H group>L group. [Conclusion] Starting from 1-day-old, feeding male broilers with doses of 100, 150 and 200 mg/(kg·BW) of gossypol for 20 consecutive days by gavage reduced growth performance, decreased serum antioxidant capacity, and caused kidney damage. With the prolongation of gossypol feeding time, the above affects were the most obvious at the dose of 150 mg/(kg·BW).

Key words: gossypol, male broiler, growth performance, serum antioxidant enzyme, renal pathological change

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