广西壮族自治区猪圆环病毒2型（PCV2）分子流行病学调查及遗传变异分析

doi:10.12160/j.issn.1672-5190.2024.02.014

畜牧与饲料科学 ›› 2024, Vol. 45 ›› Issue (2): 109-115.doi: 10.12160/j.issn.1672-5190.2024.02.014

广西壮族自治区猪圆环病毒2型（PCV2）分子流行病学调查及遗传变异分析

黄美芝¹, 何奇松², 冯淑萍², 龙凤², 尹彦文², 莫胜兰², 胡丽萍², 黄胜斌², 韩银华², 周庆安², 蓝惠华², 韦海娜², 魏园园², 甘雨², 施开创²

1.广西壮族自治区南宁市隆安县动物疫病预防控制中心,广西隆安 532700;
2.广西壮族自治区动物疫病预防控制中心,广西南宁 530001

收稿日期:2024-02-21 出版日期:2024-03-30 发布日期:2024-05-06
通讯作者: 施开创（1968—）,男,研究员,博士,主要从事动物疫病诊断与防控技术研究工作。
作者简介:黄美芝（1983—）,女,高级兽医师,主要从事动物疫病防控与检测工作。
基金资助:
广西农业农村厅科技项目（Z2022193、Z2022194、Z2022195）

Molecular Epidemiological Investigation and Genetic Variation Analysis of Porcine Circovirus Type 2 （PCV2）in Guangxi Zhuang Autonomous Region

HUANG Meizhi¹, HE Qisong², FENG Shuping², LONG Feng², YIN Yanwen², MO Shenglan², HU Liping², HUANG Shengbin², HAN Yinhua², ZHOU Qing′an², LAN Huihua², WEI Haina², WEI Yuanyuan², GAN Yu², SHI Kaichuang²

1. Animal Disease Prevention and Control Center of Long′an County of Nanning City of Guangxi Zhuang Autonomous Region,Long′an 532700,China;
2. Animal Disease Prevention and Control Center of Guangxi Zhuang Autonomous Region,Nanning 530001,China

Received:2024-02-21 Online:2024-03-30 Published:2024-05-06

摘要/Abstract

摘要： [目的]了解猪圆环病毒2型（porcine circovirus type 2,PCV2）在广西壮族自治区的流行情况及遗传特征。[方法]采用PCR方法对广西壮族自治区不同地区送检的病料进行PCV2检测和全基因组序列扩增。应用BioEdit、Mega 7.0、RDP 5和SimPlot（ver 3.5.1）软件对获得的24株PCV2毒株全基因组序列进行核苷酸序列相似性、遗传变异和重组分析,并对Cap蛋白氨基酸序列变异位点进行分析。[结果]PCV2广西株基因组大小均为1 768 bp;核苷酸序列相似性分析显示,广西株与参考株PCV1~PCV4的相似性在44.7%~99.7%,与PCV3亲缘性最低;广西株为PCV2b和PCV2d型,PCV2d流行最为广泛;全基因组序列重组分析显示,部分广西株存在重组事件;与疫苗株AY686764-PCV2b和HM641752-PCV2b相比,PCV2广西株的Cap蛋白氨基酸序列共有21个位点发生变异。[结论]PCV2广西流行毒株以PCV2d型为主,部分毒株具有重组现象,部分位点发生独特的氨基酸变异,遗传进化趋势明显。研究结果为广西壮族自治区PCV2的流行病学调查和遗传进化特征分析提供了基本数据。

关键词: 猪圆环病毒2型, 全基因组, 相似性, 遗传变异

Abstract: [Objective] This study was conducted to understand the prevalence and genetic characteristics of porcine circovirus type 2 （PCV2） in Guangxi Zhuang Autonomous Region. [Method] The clinical samples collected from different areas of Guangxi Zhuang Autonomous Region were subjected to PCV2 detection and whole genome sequence amplification by PCR assay. The bioinformatics software including BioEdit, Mega 7.0, RDP 5 and SimPlot （ver 3.5.1） were used to perform nucleotide sequence similarity, genetic variation and recombination analyses on the while genome sequences of 24 PCV2 strains obtained. In addition, the variation sites on amino acid sequences of the Cap protein were analyzed. [Result] The genomic size of the PCV2 Guangxi strains were all 1 768 bp. Nucleotide sequence similarity analysis showed that the PCV2 Guangxi strains shared 44.7% to 99.7% similarity with the referential strains PCV1-PCV4, and had the lowest similarity with PCV3. The PCV2 Guangxi strains were determined as PCV2b and PCV2d subtypes, with PCV2d being the most prevalent. Whole genome sequence recombination analysis demonstrated that there were recombination events in selected PCV2 Guangxi strains. In comparison with the vaccine strains AY686764-PCV2b and HM641752-PCV2b, a total of 21 variation sites were observed on the amino acid sequences of the Cap protein of the PCV2 Guangxi strains. [Conclusion] PCV2d was the dominant subtype of the PCV2 Guangxi strains. Several strains had genomic recombination events and unique amino acid variations on some loci, indicating a clear genetic evolution trend. The research results provided basic data for the epidemiological investigation and genetic evolution analysis of PCV2 in Guangxi Zhuang Autonomous Region.

Key words: porcine circovirus type 2, whole genome, similarity, genetic variation

中图分类号:

S852.659.2

黄美芝, 何奇松, 冯淑萍, 龙凤, 尹彦文, 莫胜兰, 胡丽萍, 黄胜斌, 韩银华, 周庆安, 蓝惠华, 韦海娜, 魏园园, 甘雨, 施开创. 广西壮族自治区猪圆环病毒2型（PCV2）分子流行病学调查及遗传变异分析[J]. 畜牧与饲料科学, 2024, 45(2): 109-115.

HUANG Meizhi, HE Qisong, FENG Shuping, LONG Feng, YIN Yanwen, MO Shenglan, HU Liping, HUANG Shengbin, HAN Yinhua, ZHOU Qing′an, LAN Huihua, WEI Haina, WEI Yuanyuan, GAN Yu, SHI Kaichuang. Molecular Epidemiological Investigation and Genetic Variation Analysis of Porcine Circovirus Type 2 （PCV2）in Guangxi Zhuang Autonomous Region[J]. Animal Husbandry and Feed Science, 2024, 45(2): 109-115.

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广西壮族自治区猪圆环病毒2型（PCV2）分子流行病学调查及遗传变异分析

Molecular Epidemiological Investigation and Genetic Variation Analysis of Porcine Circovirus Type 2 （PCV2）in Guangxi Zhuang Autonomous Region

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