蒙古羊不同尾椎数相关选择信号的挖掘研究

doi:10.12160/j.issn.1672-5190.2019.01.007

畜牧与饲料科学 ›› 2019, Vol. 40 ›› Issue (1): 28-33.doi: 10.12160/j.issn.1672-5190.2019.01.007

蒙古羊不同尾椎数相关选择信号的挖掘研究

达赖[1];付绍印[1,2];刘斌[3];何小龙[1];王标[1];陈欣[1];方勤圆[1];张兴夫[1];姜卓[4];张文广[2,3,5];刘永斌[1]

[1]内蒙古自治区农牧业科学院,内蒙古呼和浩特010031;[2]内蒙古ATCG生物信息研究所,内蒙古呼和浩特010018;[3]内蒙古犇牛科技有限公司,内蒙古呼和浩特010020;[4]扎兰屯市农牧业局畜牧工作站,内蒙古扎兰屯162650;[5]内蒙古农业大学动物科学学院,内蒙古呼和浩特010018

收稿日期:2018-11-20 出版日期:2019-01-30 发布日期:2019-08-19
通讯作者: 达赖-内蒙古自治区农牧业科学院,内蒙古呼和浩特010031
作者简介:达赖(1979—),男,副研究员,硕士,主要研究方向为绵羊生产管理;通讯作者:张文广(1973—),男,教授,博士,主要从事家畜生物信息及数量遗传机理研究工作;通讯作者:刘永斌(1977—),男,研究员,博士,主要从事绵羊遗传育种与繁殖工作。
基金资助:
“十二五”农村领域国家科技计划(2015BAD03B0505);国家肉羊产业技术体系建设项目(CARS-38);内蒙古农牧业科学院创新基金项目(2017CXJJM01-2,2018CXJJM02);内蒙古自治区科技创新引导项目(KCBJ2018054);2018年内蒙古科技计划重点项目。

Selection Signal Mining of Number of Caudal Vertebrae in Mongolian Sheep

Dalai[1];FU Shao-yin[1,2];LIU Bin[3];HE Xiao-long[1];WANG Biao[1];CHEN Xin[1];FANG Qin-yuan[1];ZHANG Xing-fu[1];JIANG Zhuo[4];ZHANG Wen-guang[2,3,5];LIU Yong-bin[1]

[1]Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences,Hohhot 010031,China;[2]Inner Mongolia ATCG Bioinformatics Institute,Hohhot 010018,China;[3]Inner Mongolia Benniu Science and Technology Co.,Ltd.,Hohhot 010020,China;[4]Animal Husbandry Work Station of Agricultural and Animal Husbandry Bureau of Zalantun,Zalantun 162650,China;[5]College of Animal Sciences,Inner Mongolia Agricultural University,Hohhot 010018,China

Received:2018-11-20 Online:2019-01-30 Published:2019-08-19

摘要/Abstract

摘要： 通过对比不同尾椎数的蒙古绵羊群体在基因组上的遗传分化水平,对全基因组选择信号、蒙古羊尾长性状的相关基因及可能的突变位点进行检测,试图解析尾椎形成的分子机制。基于全基因组重测序数据,使用两组群间的遗传分化系数(Fst)和遗传多样性比率(piratio)检测尾椎选择信号,将Fst值和piratio较大的染色体区段作为受选择候选区域。结果表明,共找到76个选择区域,这些区域分别落在了尾脂肪沉积和骨骼发育的QTL上,进一步对这些候选区域所包含的63个基因进行基因功能及通路的注释分析,富集到骨骼再生相关的Wnt和FGF信号通路,同时发现LRP6等功能候选基因。该研究确定了与尾椎数相关的受选择区域,推测不同尾椎数蒙古绵羊群体的形成可能由非基因编码区域的一个或者多个突变造成。

关键词: 蒙古羊;尾椎数;遗传分化系数;脂肪

Abstract: To reveal the molecular mechanism underlining the caudal vertebra formation,the genome-wide selection signal,the related genes of Mongolian sheep tail length traits and the possible mutation sites were detected by comparing the genetic differentiation level of Mongolian sheep population with different numbers of tail vertebrae.Based on whole genome resequencing data,the selection signal of caudal vertebra was screened with the genetic differentiation coefficient(Fst)and genetic diversity ratio(pi ratio)between the two sheep groups.A chromosome region with higher Fst and pi ratio value were selected as a candidate region.A total of 76 selected regions were found ultimately,which belonged to the QTL of tail fat deposition and skeletal development.GO and pathway analysis of the 63 genes contained in these candidate regions found the enrichment of Wnt and FGF signaling pathways related to bone regeneration,and the functional candidate genes such as LRP6 were also found.In summary,we identified the selected regions associated with the number of caudal vertebrae,suggesting that the formation of Mongolian sheep populations with different numbers of caudal vertebrae may be caused by one or more mutations in non-gene coding regions.

中图分类号:

S826.822

达赖,付绍印,刘斌,何小龙,王标,陈欣,方勤圆,张兴夫,姜卓,张文广,刘永斌. 蒙古羊不同尾椎数相关选择信号的挖掘研究[J]. 畜牧与饲料科学, 2019, 40(1): 28-33.

Dalai,FU Shao-yin,LIU Bin,HE Xiao-long,WANG Biao,CHEN Xin,FANG Qin-yuan,ZHANG Xing-fu,JIANG Zhuo,ZHANG Wen-guang,LIU Yong-bin(Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences,Hohhot 010031,China,Inner Mongolia ATCG Bioinformatics Institute,Hohhot 010018,China,Inner Mongolia Benniu Science and Technology Co.,Ltd.,Hohhot 010020,China,Animal Husbandry Work Station of Agricultural and Animal Husbandry Bureau of Zalantun,Zalantun 162650,China,College of Animal Sciences,Inner Mongolia Agricultural University,Hohhot 010018,China). Selection Signal Mining of Number of Caudal Vertebrae in Mongolian Sheep[J]. Animal Husbandry and Feed Science, 2019, 40(1): 28-33.

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蒙古羊不同尾椎数相关选择信号的挖掘研究

Selection Signal Mining of Number of Caudal Vertebrae in Mongolian Sheep

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