武定鸡脂联素基因多态性分析

doi:10.12160/j.issn.1672-5190.2020.02.005

畜牧与饲料科学 ›› 2020, Vol. 41 ›› Issue (2): 17-19.doi: 10.12160/j.issn.1672-5190.2020.02.005

武定鸡脂联素基因多态性分析

古旭¹, 丁相红², 陈粉粉²

1.西南林业大学云南生物多样性研究院,云南昆明 650224;
2.西南林业大学生命科学学院,云南昆明 650224

收稿日期:2020-02-07 出版日期:2020-02-29 发布日期:2020-04-10
通讯作者: 陈粉粉（1976—）,女,高级实验师,博士,主要研究方向为生物技术与家畜育种。
作者简介:古旭（1988—）,女,实验师,硕士,主要研究方向为生物多样性保护。
基金资助:
云南省特色优势重点学科生物学一级学科建设项目（50097505）; 云南省教育厅科学研究基金项目（2012Y230）

Analysis of Polymorphism on Adiponectin Gene of Wuding Chicken

GU Xu¹, DING Xiang-hong², CHEN Fen-fen²

1.Yunnan Institute of Biodiversity,Southwest Forestry University,Kunming 650224,China;
2.College of Life Science,Southwest Forestry University,Kunming 650224,China

Received:2020-02-07 Online:2020-02-29 Published:2020-04-10

摘要/Abstract

摘要： [目的]对武定鸡脂联素基因（NC_006096）第1外显子上的c.A99G突变位点进行多态性分析。[方法]翅静脉采集67只武定鸡全血,提取基因组DNA,采用PCR-RFLP法分析Hsp92Ⅱ限制性内切酶对PCR产物进行单酶切的结果。[结果]在c.A99G同义突变位点上存在AA、AB和BB 3种基因型,其中A基因频率为0.686 6,B基因频率为0.313 4;PIC=0.337 8,表明该位点为中度多态位点;χ²适合性检验表明,脂联素基因c.A99G位点处于平衡状态。[结论]采用PCR-RFLP法可有效检测武定鸡脂联素基因c.A99G突变,该位点有3种基因型,等位基因呈中度遗传多态水平且处于平衡状态。

关键词: 武定鸡, 脂联素基因, PCR-RFLP

Abstract: [Objective] To analyze the polymorphism of c.A99G mutation site on exon 1 of adiponectin gene （NC_006096） of Wuding chicken. [Method] The whole blood samples of 67 Wuding chickens were collected from the wing veins, and the genomic DNA was extracted. The PCR products digested with Hsp92Ⅱ restriction enzyme were analyzed via PCR-RFLP assay. [Result] Three genotypes of AA, AB and BB were observed at the synonymous mutation site of c.A99G, in which the frequency of A gene was 0.686 6 and that of B gene was 0.313 4; the PIC was 0.337 8, indicating that the site was a moderate polymorphic site;χ² fitness test showed that the adiponectin gene c.A99G site was in equilibrium. [Conclusion] The PCR-RFLP method effectively detected the c.A99G mutation of adiponectin gene of Wuding chicken. There were 3 genotypes at this locus, and the alleles were at the level of moderate genetic polymorphism and were in equilibrium.

Key words: Wuding chicken, adiponectin gene, PCR-RFLP

中图分类号:

S831.2

古旭, 丁相红, 陈粉粉. 武定鸡脂联素基因多态性分析[J]. 畜牧与饲料科学, 2020, 41(2): 17-19.

GU Xu, DING Xiang-hong, CHEN Fen-fen. Analysis of Polymorphism on Adiponectin Gene of Wuding Chicken[J]. Animal Husbandry and Feed Science, 2020, 41(2): 17-19.

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武定鸡脂联素基因多态性分析

Analysis of Polymorphism on Adiponectin Gene of Wuding Chicken

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