FAD3基因编辑小鼠内脏重量、肌肉中脂肪酸组成与含量以及盲肠菌群分布

doi:10.12160/j.issn.1672-5190.2020.03.004

畜牧与饲料科学 ›› 2020, Vol. 41 ›› Issue (3): 16-21.doi: 10.12160/j.issn.1672-5190.2020.03.004

FAD3基因编辑小鼠内脏重量、肌肉中脂肪酸组成与含量以及盲肠菌群分布

韩红燕¹, 顾青¹, 王超²

1.内蒙古大学草原家畜生殖调控与繁育国家重点实验室,内蒙古呼和浩特 010070;
2.内蒙古自治区农牧业科学院,内蒙古呼和浩特 010031

收稿日期:2020-01-17 出版日期:2020-05-30 发布日期:2020-06-17
通讯作者: 王超（1980—）,助理研究员,博士,主要从事饲料微生物与动物营养研究工作。
作者简介:韩红燕（1984—）,讲师,博士,主要从事动物营养研究工作。
基金资助:
内蒙古自然科学基金面上项目（2018MS03076）；内蒙古农牧业科技创新基金项目（2017CXJJM04）。

Visceral Weight, Composition and Content of Muscular Fatty Acid, and Cecum Microflora Distribution in FAD3 Gene-edited Mouse

HAN Hong-yan¹, GU Qing¹, WANG Chao²

1.The State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock,Hohhot 010070,China;
2.Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences,Hohhot 010031,China

Received:2020-01-17 Online:2020-05-30 Published:2020-06-17

摘要/Abstract

摘要： 为了解析外源基因的转入对动物生长特性、组织中脂肪酸含量和肠道菌群分布的影响,该研究以FAD3基因编辑小鼠为试验组,以BDF1小鼠为对照组,在相同条件下饲养至45日龄处死,检测两组小鼠体重、各器官重量、肌肉中不饱和脂肪酸含量,并利用PCR-DGGE方法在16S rDNA的V₃领域检测小鼠盲肠菌群分布。结果显示,FAD3组小鼠肝脏重量显著（P<0.05）低于对照组,其他各内脏器官重量和体重两组均无显著（P>0.05）差异。FAD3组小鼠肌肉中n-6多不饱和脂肪酸（PUFAs）含量低于对照组,但无显著（P>0.05）差异,而n-3 PUFAs含量显著（P<0.05）高于对照组。两组小鼠盲肠菌群分布个体差异大,未观测到明显的组间差异,Anaerotaenia torta为共同的优势菌,而Flavonifractor plautii可能为FAD3组小鼠特有菌。该试验结果提示,外源基因FAD3的引入对小鼠生长性能和盲肠菌群的影响较小,可提升肌肉中n-3多不饱和脂肪酸含量。

关键词: FAD3基因, 转基因小鼠, 盲肠菌群, 不饱和脂肪酸

Abstract: The aim of this study was to assess the effects of transferring foreign genes on the growth characteristics, fatty acid content in the tissues and the distribution of intestinal microflora of mouse. The FAD3 gene-edited mice were used as the experimental group and the BDF1 mice were used as the control group. The mice were reared in the same condition and were killed at 45 days of age. The body weights, organ weights and unsaturated fatty acid contents in the muscles of the two groups of mice were measured, and the distribution of the cecum microflora was detected by PCR-DGGE targeting at the V₃ variable region of 16S rDNA. The results showed that the liver weight of the mice in the FAD3 group was significantly （P<0.05） lower than that in the control group, and there was no significant （P>0.05) difference in the weights of other organs and body weights between the two groups. The content of muscular n-6 polyunsaturated fatty acids （n-6 PUFAs） of the FAD3 group was lower than that of the control group, but there was no significant （P>0.05） difference; while the content of muscular n-3 PUFAs of the FAD3 group was significantly （P<0.05） higher than that of the control group. The cecum flora distribution of the two groups of mice displayed obvious individual differences, and no significant difference in cecum flora distribution was observed between the two groups. Anaerotaenia torta was a common dominant bacterial species of the two groups, while Flavonifractor plautii was probably a strain unique to the FAD3 group. The results suggest that the introduction of the foreign gene FAD3 has minor effects on growth performance and cecum microflora in mouse, and can increase the content of n-3 PUFAs in mouse muscle.

Key words: FAD3 gene, transgenic mouse, cecum microflora, unsaturated fatty acids

中图分类号:

Q343.15

韩红燕, 顾青, 王超. FAD3基因编辑小鼠内脏重量、肌肉中脂肪酸组成与含量以及盲肠菌群分布[J]. 畜牧与饲料科学, 2020, 41(3): 16-21.

HAN Hong-yan, GU Qing, WANG Chao. Visceral Weight, Composition and Content of Muscular Fatty Acid, and Cecum Microflora Distribution in FAD3 Gene-edited Mouse[J]. Animal Husbandry and Feed Science, 2020, 41(3): 16-21.

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FAD3基因编辑小鼠内脏重量、肌肉中脂肪酸组成与含量以及盲肠菌群分布

Visceral Weight, Composition and Content of Muscular Fatty Acid, and Cecum Microflora Distribution in FAD3 Gene-edited Mouse

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