胎盘提取物的制备与免疫调节功能及应用研究进展

doi:10.12160/j.issn.1672-5190.2020.05.014

摘要/Abstract

摘要： 主要介绍了胎盘提取物的制备方法、免疫调节功能以及在生产生活中的应用。总结出, 胎盘提取物有着免疫调节、抗氧化、抗肿瘤和营养作用;其制备方法主要有超滤法、酶解法和微生物发酵法, 各自具有优势和不足, 选择胎盘提取物制备方法时可以根据自有的条件和要求及前人的经验综合判断;在皮肤护理方面, 胎盘提取物可以作为抗氧化成分添加到护肤品中以起到延缓皮肤衰老的作用;在疫病治疗方面, 能改善肝炎患者的临床症状和肝功能, 对胰腺癌细胞的增殖有负调控作用, 对于超敏反应也有一定的治疗效果, 在神经再生方向效果显著。胎盘提取物在保健品和护肤品研发、疾病治疗方面应用前景广阔。

关键词: 胎盘, 提取物, 制备方法, 免疫调节

Abstract: In this paper, the preparation methods, immunomodulatory functions and applications of placenta extracts in life and production are introduced. The placenta extracts have immunomodulatory, anti-oxidative, anti-neoplastic and nutritional functions. The preparation methods of placenta extracts mainly include ultra-filtration method, enzymatic hydrolysis method and microbial fermentation method. Each method has its advantages and disadvantages. Accordingly, when choosing the preparation method of placenta extracts, comprehensive consideration should be taken according to the own conditions and requirements as well as previous experience. In the aspect of skin care, placenta extracts can be added as an antioxidant component to skin care products to delay skin aging. In terms of disease treatment, placenta extracts can improve the clinical symptoms and liver function of patients with hepatitis, have a negative regulatory effect on the proliferation of pancreatic cancer cells, show a certain therapeutic effect on hypersensitivity, and exhibit a significant effect in nerve regeneration. Placenta extracts have broad application prospects in research and development of health care products, skin care products as well as in disease treatment.

Key words: placenta, extracts, preparation methods, immunomodulation

中图分类号:

赵素洵, 玉斯日古楞, 云皓琪, 么宏强. 胎盘提取物的制备与免疫调节功能及应用研究进展[J]. 畜牧与饲料科学, 2020, 41(5): 75-79.

ZHAO Su-xun, Yusiriguleng, YUN Hao-qi, YAO Hong-qiang. Research Advances on Preparation Methods, Immunomodulatory Functions and Applications of Placenta Extracts[J]. Animal Husbandry and Feed Science, 2020, 41(5): 75-79.

参考文献

[1] ZAMUDIO S.Epidemiological, clinical and molecular insights into the pathophysiology of Abnormally Invasive Placenta (AIP)[J].Placenta, 2019, 83:e3.
[2] CARTER A M.Fetal membranes - A focus for the future or a legacy from the past?[J].Placenta, 2015, 36(9):A2.
[3] SALAS L, DURAN E J, BENAVIDES O S, et al.Estimation of fetal radiation dose during the prophylactic use of the aortic occlusion balloon on abnormally invasive placenta pregnancies[J].Placenta, 2019, 83:e102.
[4] PÉREZ-PÉREZ A, VILARIÑO-GARCÍA T, RIEDEL R, et al. Role of leptin on the apoptosis of trophoblast explants triggered by acidosis[J].Placenta, 2019, 83:e102.
[5] WU X P, YANG Z F, LI Z H, et al.Expression levels and significance of hypoxia inducible factor-1 alpha and vascular endothelial growth factor in the gastric mucosa of streptozotocin-induced diabetic rats[C]//中国解剖学会2019年年会论文文摘汇编.上海:《解剖学杂志》编辑部, 2019:156.
[6] DUBOVA E A, PAVLOV K A, LYAPIN V M, et al.Expression of insulin-like growth factors in the placenta in preeclampsia[J].Bulletin of Experimental Biology and Medicine, 2014, 157(1):103-107.
[7] 蒙怡. 胎盘免疫调节因子的制备及其对CIK细胞活性影响的研究[D].南宁:广西医科大学, 2007.
[8] 刘隆兴, 任兴宏, 汤禾静, 等.羊胎盘免疫活性小分子肽的分离纯化[J].食品科学, 2013, 34(22):273-276.
[9] 郭金刚. 利用胎盘制备转移因子[J].江苏医药, 1979(Z1):58.
[10] 刘月新, 王勋楚, 段茂芳, 等.一种新的免疫调节剂——胎盘因子的制备与研究[J].中国免疫学杂志, 1985(5):51-54.
[11] 周国华, 孟凡军, 王永志, 等.羊和猪胎盘肽的提取[J].动物医学进展, 2005, 26(9):88-89.
[12] 马兴亮. 奶山羊胎盘肽的分离提纯及其对小白鼠免疫功能的影响[D].泰安:山东农业大学, 2008.
[13] 高闪闪. 奶山羊胎盘抗氧化多肽制备及其功能活性研究[D].杨凌:西北农林科技大学, 2017.
[14] 刘辉. 羊胎盘多肽的制备工艺及抗氧化活性研究[D].长春:吉林大学, 2018.
[15] 皮钰珍, 刘长江, 王淑琴, 等.双酶水解法制备鹿胎盘活性多肽[J].食品与发酵工业, 2006(9):168-171.
[16] MALTEPE E, FISHER S J.Placenta: the forgotten organ[J].Annual Review of Cell and Developmental Biology, 2015, 31:523-552.
[17] ZHONG N, ZHONG M.China Human Placenta Project: A global effort to promote placenta medicine[J].Placenta, 2016, 44:112-113.
[18] 朱蓓薇, 葛瑞宏.双酶水解法制备羊胎盘活性肽[J].食品与发酵工业, 2003(11):41-45.
[19] 房新平. 牛胎盘免疫活性肽提取与酶法制备研究[D].无锡:江南大学, 2007.
[20] 王顺新. 酶法水解牛胎盘下脚料的工艺研究[J].食品安全导刊, 2016(9):93-95.
[21] 巴达马其其格, 武晓东, 侯霞, 等.鹿胎盘生物活性多肽的制备及其药理作用研究[J].内蒙古农业大学学报(自然科学版), 2008, 29(3):4-8.
[22] 李娇娇. 鹅骨抗氧化肽的酶解工艺优化及其分离纯化的研究[D].雅安:四川农业大学, 2013.
[23] 李硕, 赵晓云, 常世杰, 等.酶解法和超滤法获得羊胎盘提取物对人脐静脉内皮细胞增殖影响差异[J].解剖科学进展, 2011, 17(6):548-551.
[24] 张华. 羊胎盘免疫调节因子对小鼠非特异性免疫功能影响的初步研究[D].乌鲁木齐:新疆农业大学, 2005.
[25] 刘旺旺, 侯银臣, 程永霞, 等.羊胎盘多肽的制备及其清除自由基能力的研究[J].中国酿造, 2014, 33(9):89-93.
[26] 侯银臣, 吴丽, 刘旺旺, 等.羊胎盘免疫活性肽的制备及其活性[J].中国食品学报, 2016, 16(1):123-129.
[27] 黄楚华, 殷学伦.胎盘肽研究的进展[J].中国生化药物杂志, 1994(4):294-296.
[28] 钟英英. 羊胎盘免疫调节因子调节BALB/c小鼠细胞免疫功能的实验研究[D].重庆:西南师范大学, 2005.
[29] 刘佳. 骆驼胎盘干粉对小鼠免疫功能影响的研究[D].呼和浩特:内蒙古农业大学, 2014.
[30] 王炳祥. 山羊胎盘肽的分离和初步鉴定及营养、免疫、抗氧化作用的研究[D].泰安:山东农业大学, 2011.
[31] 宁鹏. 牦牛胎盘免疫调节因子对小鼠免疫器官指数及组织结构的影响[J].安徽农业科学, 2019, 47(9):90-92.
[32] LEE K H, PARK H J, SEO H G, et al.Immune modulation effect of porcine placenta extracts in weaned the pig[J].Journal of Animal Science, 2013, 91(5):2405-2413.
[33] PHONCHAI R, UBONSAEN B, BRAMELD S, et al.Case report: improvement of long lasting hyperpigmentation by aqueous human placenta extract (reju growth factor, RGF) treatment[J].Journal of Cosmetics, Dermatological Sciences and Applications, 2019, 9:125-128.
[34] MITSUI Y, BAGCHI M, MARONE P A.et al.Safety and toxicological evaluation of a novel, fermented, peptide-enriched, hydrolyzed swine placenta extract powder[J].Toxicology Mechanisms and Methods, 2015, 25(1):13-20.
[35] JUNG J, LEE H, LEE J M, et al.Placenta extract promote liver regeneration in CCl₄-injured liver rat model[J].International Immunopharmacology, 2011, 11(8):976-984.
[36] LOBO S E, LEONEL L C P C, MIRANDA C M F C, et al. The placenta as an organ and a source of stem cells and extracellular matrix: a review[J].Cells Tissues Organs, 2016, 201:239-252.
[37] 王晶, 林志颖, 林立梅, 等.牛羊胎盘免疫调节因子的提取及对PANC-Ⅰ增殖和迁移影响的比较[J].农业生物技术学报, 2016, 24(12):1863-1871.
[38] NARDID O, REPINA S, BOBROVA E, et al.Beneficial impact of human placenta extracts on erythrocyte membrane thermostability[J].Trakia Journal of Sciences, 2018, 16(3):204-211.
[39] JASH A, KWON H, SAHOO A, et al.Topical application of porcine placenta extract inhibits the progression of experimental contact hypersensitivity[J].Journal of Ethnopharmacology, 2011, 133(2):654-662.
[40] KOGURE C, TOHDA C.Human placenta extract ameliorates memory dysfunction and dendritic atrophy in a 5XFAD mouse model of Alzheimer′s disease[J].Traditional and Kampo Medicine, 2017, 4(2):94-104.