Abstract: A total of 120 AA commercial broilers were subjected to low temperature stress with different decreasing degree of temperature from 22 to 42 days of age.The rearing temperature of the experimental groups was 3,5,7 ℃ lower than that of the control group,and the cold stimulation period all lasted 3 hours for both experimental groups and control group.After the cold stimulation,all of the experimental broilers were reared at the normal temperature.The behavior indexes,including lying,feeding,standing,drinking,walking and grooming were used to evaluate the impact of low temperature stress on brooding of AA broiler.The results showed that cold stimulation with decreasing temperature had significant impact on walking（P0.05） of AA broiler but had no significant impact on feeding,lying,grooming,drinking and standing（P0.05）.