Abstract: Currently, Cell Engineering is one of the main courses for undergraduates majoring in biotechnology, bio-engineering and biological science in colleges and universities. This course is a practical and highly applied discipline and involves a large number of experimental operating techniques. Therefore, the fact that combining the theoretical teaching with practice and promoting them mutually is of great significance. In this teaching mode, the practice will be brought to theoretical teaching and the theoretical teaching will be brought to practice, they are organically combined, so that the good teaching effect will be achieved.The significance of the combination of theoretical teaching and practice in Cell Engineering is elaborated, and the ways and methods for teaching mode reform of this course are summarized.