Abstract: Livestock breeding at home and abroad is developing rapidly.New theories and research results in this discipline are emerging.The novel methods and technologies are playing increasingly important roles in livestock breeding production.Accordingly,the content of Livestock Breeding is also constantly enriched.In the teaching process of Livestock Breeding in College of Animal Science and Technology of Henan University of Science and Technology,there is lack of comprehensive and practical experiment.Furthermore,the undergraduates lack interests in practical operating in the experiment course.In view of the above problems,the GBS breeding and analysis system is applied in the experimental teaching of Livestock Breeding,which is embodied in single breeding value estimation,multi-trait breeding value estimation,inbreeding coefficient calculation,selection plan and so on.After the implementation of the above experimental teaching reform,the enthusiasm of the undergraduates′participation in experimental course of Livestock Breeding has been improved and their professional skills have been enhanced.