Abstract: [Objective]To study the effects of ZD preparation on the concentration of TNF-α in a cellular model of inflammation and the death of mouse fibroblast L929 cells induced by TNF-α. [Methods] The mouse peritoneal macrophage RAW264.7 cell line was stimulated by lipopolysaccharide（LPS） to establish a cellular model of inflammation, and five different concentrations of ZD preparation（100, 10, 1, 0.1, 0.01 μg/m L） were included in this study. ELISA method was used to detect the TNF-αconcentration in the cell supernatant before and after ZD preparation intervention. The cytotoxic model was established with mouse fibroblast L929 cell line by stimulation of TNF-α, and the activity of L929 cells was determined by MTT method before and after the intervention of ZD preparation. [Results] The 100, 10, 1 and 0.1 μg/m L of ZD preparation groups had extremely significant difference in TNF-α concentration compared to the LPS model group（P〈0.01）, and the 0.01 μg/m L of ZD preparation group showed significant difference in TNF-α concentration compared to the LPS model group（P〈0.05）. In addition, the activity of mouse fibroblast L929 cells were significantly elevated after the intervention of 100 and 10 μg/m L of ZD preparation compared to the TNF-α group. [Conclusion] ZD preparation exerts the anti-inflammatory effects via reducing the secretion of TNF-α and inhibiting the biological activity of TNF-α.