鸡血藤总黄酮对环磷酰胺所致小鼠肝损伤的保护作用

doi:10.12160/j.issn.1672-5190.2022.04.002

摘要/Abstract

摘要：

[目的]研究鸡血藤总黄酮对环磷酰胺所致小鼠肝损伤的保护作用。[方法]将60只昆明系小鼠随机分为6组，每组10只，试验周期为7 d。对照组小鼠试验全程给予生理盐水。环磷酰胺（CTX）组小鼠1~4 d灌胃给予生理盐水；5~7 d腹腔注射80 mg/（kg·BW）CTX，每天给药1次。鸡血藤总黄酮（TFSD）100组、CTX+TFSD25组、CTX+TFSD50组、CTX+TFSD100组小鼠1~7 d分别灌胃给予100、25、50、100 mg/（kg·BW）TFSD，每天给药1次；试验5~7 d除TFSD100组小鼠腹腔注射生理盐水外，其余试验组腹腔注射80 mg/（kg·BW）CTX，每天给药1次。试验结束后对各组小鼠进行眼球采血及剖检；检测肝组织中超氧化物歧化酶（SOD）、髓过氧化物酶（MPO）、黄嘌呤氧化酶（XOD）以及谷胱甘肽过氧化物酶（GSH-Px）活力，测定血清中谷草转氨酶（AST）、谷丙转氨酶（ALT）和碱性磷酸酶（ALP）活力，检测血清中肿瘤坏死因子-α（TNF-α）、白介素-6（IL-6）和白介素-1β（IL-1β）含量。[结果]环磷酰胺可显著（P<0.05）降低小鼠肝脏中SOD、GSH-Px以及血清中ALT活力，显著（P<0.05）升高肝脏中XOD与MPO活力、血清中ALP活力以及炎症细胞因子IL-1β和IL-6释放水平。25、50、100 mg/（kg·BW）的TFSD处理均可显著（P<0.05）抑制由CTX引起的SOD活力降低，25 mg/（kg·BW）的TFSD处理可显著（P<0.05）提高CTX处理小鼠的GSH-Px活力；50 mg/（kg·BW）的TFSD处理可显著（P<0.05）抑制由CTX引起的XOD和MPO活力升高，且可显著（P<0.05）降低CTX处理小鼠血清中的AST活力；25、50、100 mg/（kg·BW）的TFSD处理可显著（P<0.05）抑制由CTX引起的血清中ALP活力升高，同时降低ALT活力。25、50、100 mg/（kg·BW）的TFSD处理均可显著（P<0.05）抑制由CTX引起的IL-1β水平升高。[结论]鸡血藤总黄酮可通过调节小鼠炎症因子释放以及肝组织中氧化还原酶水平对环磷酰胺所致肝损伤提供保护，推荐剂量为25 mg/（kg·BW）。

关键词: 鸡血藤总黄酮, 环磷酰胺, 肝损伤, 炎症因子, 氧化还原酶

Abstract:

[Objective] To evaluate the protective effects of total flavonoids from Spatholobus suberectus Dunn （TFSD） on liver injury induced by cyclophosphamide （CTX） in mice.[Method] A total of 60 Kunming mice were randomly divided into 1 of 6 groups with 10 mice in each group. The experimental period lasted for 7 days. The control group received physiological saline throughout the whole experiment. The CTX group was gavaged with physiological saline from 1^st to 4^th day of the experiment, and was intraperitoneally injected with 80 mg/（kg·BW） CTX once a day from 5^th to 7^th day of the experiment. TFSD100 group, CTX+TFSD25 group, CTX+TFSD50 group and CTX+TFSD100 group were gavaged with 100, 25, 50 and 100 mg/（kg·BW） TFSD once a day from 1^st to 7^th day of the experiment, respectively. From 5^th to 7^th day of the experiment, all the other experimental groups received intraperitoneal injections of 80 mg/（kg·BW） CTX once a day, with the exception of the TFSD100 group, which received intraperitoneal injections of physiological saline. At the end of the experiment, the mice in each group were sacrificed and their blood samples were taken from the eyeballs. The activities of superoxide dismutase （SOD）, myeloperoxidase （MPO）, xanthine oxidase （XOD）, and glutathione peroxidase （GSH-Px） in liver tissues were tested. In addition, the serum activities of aspartate aminotransferase （AST）, alanine aminotransferase （ALT） and alkaline phosphatase （ALP） as well as the serum levels of tumor necrosis factor-α （TNF-α）, interleukin-6 （IL-6） and interleukin-1β （IL-1β） were measured. [Result] CTX significantly （P<0.05） decreased the activities of SOD and GSH-Px in liver tissues and the serum activity of ALT, while significantly （P<0.05） elevated the activities of XOD and MPO in liver tissues, the serum activity of ALP, and the serum levels of IL-1β and IL-6. The treatments of 25, 50 and 100 mg/（kg·BW） TFSD all significantly （P<0.05） inhibited the decrease of SOD activity induced by CTX, and 25 mg/（kg·BW） TFSD significantly （P<0.05） elevated the GSH-Px activity in CTX treated mice. The treatment of 50 mg/(kg·BW) TFSD significantly （P<0.05） inhibited the elevation of XOD and MPO activities induced by CTX, and significantly （P<0.05） reduced the AST activity in CTX treated mice. The treatments of 25, 50 and 100 mg/（kg·BW） TFSD all significantly （P<0.05） inhibited the increase of ALP activity induced by CTX, and reduced the ALT activity. The treatments of 25, 50 and 100 mg/（kg·BW） TFSD all significantly （P<0.05） inhibited the increase of IL-1β level induced by CTX. [Conclusion] TFSD exhibited protective effects on cyclophosphamide induced liver injury by regulating the release of inflammatory factors and the levels of oxidoreductase in liver tissues in mice. The recommended dose of TFSD was 25 mg/（kg·BW）.

Key words: total flavonoids from Spatholobus suberectus Dunn, cyclophosphamide, liver injury, inflammatory factor, oxidoreductase

中图分类号:

S853.74

史鑫锐, 陈滢锴, 陈中婷, 赵尉丹, 江明生, 陈海兰. 鸡血藤总黄酮对环磷酰胺所致小鼠肝损伤的保护作用[J]. 畜牧与饲料科学, 2022, 43(4): 8-13.

SHI Xin-rui, CHEN Ying-kai, CHEN Zhong-ting, ZHAO Wei-dan, JIANG Ming-sheng, CHEN Hai-lan. Protective Effects of Total Flavonoids from Spatholobus suberectus Dunn on Liver Injury Induced by Cyclophosphamide in Mice[J]. Animal Husbandry and Feed Science, 2022, 43(4): 8-13.

图/表 4

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