Abstract: The proteolytic complex of spleen and brain protein is material transfer factor carrying the allergic information released by T lymphocytes and is also brain-specific nutrient drugs of peptidergic nerve.It can enhance immunity of animals and disease resistance of young animals.The preparation methods of the proteolytic complex of spleen and brain protein were introduced.And in clinic,it was applied in Tibetan pigs at the age of 20-30 days old from a pig farm in Linzhi area of Tibetan region.The 33 piglets of the experiment group were administrated with the proteolytic complex at a dosage of 2 mL/（kg·bw）.There were 30 piglets in the control group.The results showed that the piglets in the experiment group had significantly stronger disease resistance and higher survival rate than the control group（P0.05）.