Animal Husbandry and Feed Science ›› 2010, Vol. 31 ›› Issue (11): 6-6.doi: 10.12160/j.issn.1672-5190.2010.11.003

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Effects of Diluents Added Polyunsaturated Fatty Acids(PUFA) on Frozen Sheep Semen

YANG Jian,LIU De-quan,WANG Li-fang,RONG Wei-heng(1.Agricultural and Animal Husbandry Bureau of Hohhot City,Hohhot 010020,China;2.Livestock Improvement Station of Qingshuihe County,Qingshuihe 012000,China;3.Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences,Hohhot 010031,China)   

  • Online:2010-11-20 Published:2010-11-20

Abstract: The novel additive was composed of glucose-citrate-yolk supplemented with deep-seal fish oil which is rich in polyunsaturated fatty acids(PUFA) and contains high-proportion DHA and EPA.This additive was added to frozen sheep semen to study the excellent protection effects of PUFA on sperm in freezing-thawing process.After the freezing-thawing process,the frozen sheep semen in diluents added PUFA at a proportion of 3% and VE at a proportion of 1% were damaged little.After thawing process,viability and acrosomal integrity were 6% and 10% higher than that of the control group 1(0)(P0.05),respectively.The activity of LDH enzyme(Lactate dehydrogenase) and GOT enzyme(glutamate oxaloacetate transaminase) in seminal plasma was 65% and 15% lower than that of the control group 1(0)(P0.05).

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