Abstract: In recent years, the pregnancy rates of yellow cattle and water buffalos in many cattle breed improvement stations of Xiangzhou County of Guangxi region were 33%-51% and 35%-55%, respectively, which indicated the pregnancy rate still needed to be increased. Human chorionic gonadotropin （HCG） can promote follicular development and ovulation in cows. Cows were intramuscularly injected with HCG during estrus and then artificially inseminated, and its effects on pregnancy rate and artificial insemination of cows were observed. The results showed that HCG can significantly increased pregnancy rate in artificially inseminated cattle as well as work efficiency.