The study was aimed to investigate effects of Xiaogan rice wine on memory and antioxidation function of mice.D-galactose-induced aging mice were lavaged Xiaogan rice wine at high,mediate and low concentration,respectively.After 30 d,one-trial passive avoidance response models were used to observe changes in mouse behavior,and superoxide dismutase（SOD） activity and malondialdehyde（MDA） content in brain,blood and liver were determined.The results showed that all mice had obviously shortened response time（P〈0.01）.The academic record of mice lavaged low-concentration Xiaogan rice wine was increased obviously,and they had longer incubation period,significantly higher SOD activity in brain,red cells and liver（P〈0.01）,and significantly lower MDA content in brain,serum and liver（P〈0.01）.

[Objective] To increase sporulation rate and spore concentration and offer the theoretical foundation for industrial production of Bacillus megaterium preparation. [Method] Fermentation culture medium and culture conditions of Bacillus megaterium JD-2 were studied by single factor test and orthogonal test. [Result] The optimum medium formula was composed of 10.0 g/L corn starch, 5.0 g/L peptone, 5.0 g/L yeast extract, 5.0 g/L NaCl, 1.0 g/L CaCO3 and 0.5 g/L MgSO4·7H2O. The optimum culture conditions are as follows： initial spore concentration, 10^6 CFU/mL; initial pH value, 7.0; culture temperature, 30 °C; shaking speed, 200 r/min; and volume, 25 mL medium in a 250 mL flask. When enlarge fermentation was performed in a 15 L auto fermentor with dissolved oxygen level less than 30%, cell concentration reached 3.50×10^9 CFU/mL, spore concentration reached 3.40×10^9 CFU/mL and sporulation rate reached 97.2% after 22 h culture. [Conclusion] The optimal culture conditions can be applied in practical production.

The study was aimed to observe poisonous side reaction after using Radix glycyrrhizae with Radix knoxiae of the Chinese ＂anti-18＂. The mice were assigned into five groups. The mice in the group Ⅰ, Ⅱ, Ⅲ, Ⅳ, Ⅴand CK were fed Radix glycyrrhizae decoction, Radix knoxiae decoction, dividual decoction of Radix glycyrrhizae and Radix knoxiae, admixture decoction of Radix glycyrrhizae and Radix knoxiae, and physiological saline, respectively, for 3 d. Spontaneous activity and fatality were recorded. The mice were executed after 72 h, and blood was collected for determination of glutamic-pyruvic transaminase level. Pathological section was prepared, and routine blood, liver/body ratio and kidney/body ratio was determined. The results showed that Radix knoxiae had a certain impact on liver function. Radix knoxiae compatible with Radix glycyrrhiza aggravated the impact on liver function but had no impact on kidney function. It also had effects on forms of liver and kidney organization, but they could recover after drug withdrawal.

Regression between litter size and parity in fat-tailed sheep was analyzed using SPSS software. The quadratic regression and cubic regression goodness of fit （R2） of litter size and parity was high in fat-tailed sheep, 0.924 6 and 0.926 9, respectively. All regression parameters of quadratic regression equation between litter size and parity reached highly significant level （P〈0.01）, which showed the quadratic regression equation between litter size and parity has a certain application value.

Considering advantages of night grazing and lifetime-limited grazing, grazing was studied during night instead of daytime. Adult white cashmere goats had 5.33 kg higher weight gain and 0.41 μm thicker cashmere fineness in the night grazing way than in the traditional grazing, which indicated the night grazing way had significant promoting effect on weight gain but had negative impact on fineness of white cashmere goats.

Free gossypol content in cottonseed meals （Free gossypol content of 200 -1 000 mg/kg） was determined by AOCS recommended Ba7-58 method and national standard GB 13086 method. Referring to AOCS Ba7-58, standard curve was established, and calculate factor F1 was obtained. Rate of recovery was also determined, the free gossypol content in three cottonseed meals having different free gossypol content was determined. According to AOCS Ba7-58 standard curve, F1=0.370, R2=0.999, and rate of recovery= 101.14%. According to mass absorption coefficients in GB 13086 method, the free gossypol content, rate of recovery and RSD were 62.5 L/（cm·g）, 104.98% and 9.85, respectively. The results showed gossypol and its derivates could be fully extracted by the AOCS Ba7-58 method, and the detection was stable and repeatable. Gossypol and its derivates could not be extracted stably by the GB 13086 method, and the detection had poor repeatability. Therefore, we recommended AOCS Ba7-58 method be substituted by GB 13086 method to detect free gossypol content in cottonseed meals in feed industry, and the results can accurately reflect toxic content in cottonseed meals and feed.

Root characters of four alfalfa varieties in greenhouse were compared. WL323 alfalfa had the longest main root （48.46 cm）. American Alfalfa King had longer diameter of main root, more secondary roots as well as larger root biomass and root volume than other three varieties. Any two indexes of root （diameter of main root, length of the thickest secondary root, diameter of the thickest secondary root, number of secondary roots, root biomass and root volume） except length of main root were positively correlated （significantly or extremely significantly）.

According to the population distribution characteristics of halophytes and soil salinity analysis in the Tumochuan Plain, the dominance of spatial distribution of salt-tolerant plants increased with the increasing soil salinity, and the ionic structure in plants was related to that in soil. The plants like Suaeda glauca Bge., Saussurrea salsa （Pall.） Spreng. and Artemisia anethifolia Web. which had higher concentration of K＋＋ Na＋ and Cl- tended to have stronger salt tolerance. The salt content in plants increased with that in soil, and the amplitude of salt content in plant was larger than that in soil. The plants having stronger salt tolerance had larger amplitude of salt content, which indicated plants could enrich corresponding ions in soil.

Forage production was compared between Atriplex canescens which was cut once, twice, thrice and four times in a year. The results showed that the Atriplex canescens that was cut twice or thrice had higher grass yield per plant, 2 713.2 and 2 458.7 g, respectively. And the grass yield of Atriplex canescens which was cut twice was 2.3 times as high as that of Atriplex canescens which was cut once and 1.5 times as high as that of Atriplex canescens which was cut four times. The Atriplex canescens which was cut twice or thrice had high hay yield, and the former had the highest hay yield （1 148.5 g per plant）. With increasing cutting frequency, ratio of fresh weight to dry weight was also increased, and it decreased in such order： four times cutting〉 thrice cutting 〉twice cutting〉 once cutting.

The study was aimed to study the effects of corn flour and king grass at different supplement dosage on fermentation quality of tropical Leucaena silage. The results showed that the Leucaena silage supplemented corn flour had better fermentation quality, which was similar to that of Leucaena control. Considering the quality of silage fermentation and actual production, the optimum supplement dosage of corn flour was 10% in Leucaena silage.

According to the demand of talents training, the experimental teaching system in the center of animal technology experimental teaching was improved, and an integrated multi-layer open experimental teaching system was constructed. The practice showed that the new experimental teaching system has well stimulated students′ interest in learning, enhances their ability of research and innovation and increases utilization rate of laboratory equipments.

According to course characteristics and combining with the actual situation, scene association method, formula mnemonics, and inductive and summary method together with multimedia teaching and blackboard teaching were used in teaching of animal anatomy, histology and embryology. Later stage courses were also contacted in order to broaden students′ knowledge and improve the teaching effect of animal anatomy, histology and embryology.

To stimulate students′ interest in learning Animal Anatomy course and improve the teaching effect, the teaching content, teaching methods and assessment methods were reformed in teaching practice. Recent progress and new technologies were introduced in teaching content, and mechanical memory was substituted by understanding memory. Multimedia technology was comprehensively applied to enhance teaching intuitiveness. Multi -form multi -level assessment methods were used to stimulate learning motivation. Through a variety of reforms, students′ interest and motivation in learning were enhanced, and teaching quality was also improved.

Considering the present situation of the practice teaching of Traditional Chinese Veterinary Medicine course as well as very abstract theory, complex content and disconnection between practice and teaching in teaching process of Traditional Chinese Veterinary Medicine, practice teaching was reformed to achieve integration of theory teaching and practice teaching, make abstract and complex theory intuitive and systematic, and help students understand and master theory, professional knowledge and practical skills in Traditional Chinese Veterinary Medicine.

Application of ＂task-driven＂ method in teaching can effectively enhance students′ practical ability and creative ability and improve classroom teaching. This paper describes the necessity to implement the ＂task -driven＂ method in teaching of Animal Microbiology. And combining with specific teaching cases, the application process of ＂task-driven＂ method in Animal Microbiology teaching was introduced. The teaching effect and experience were also summed up.

In order to provide practical basis for raising and breeding of Lemur catta, their biological characteristics as well as environment, behavior, nutrition, breeding and artificial rearing in artificial breeding were studied with the Lemur catta in Shijiazhuang Zoo as research objects.

[Objective] To select the best ultrasonic extraction process for separating effective ingredients from Zengye Chengqi decoction. [Method] Ultrasonic power, extraction temperature and extraction time at three levels were compared by orthogonal design test, and the optimum process was selected based on recovery rate of main effective ingredients. [Result] The best conditions are as follows： ultrasonic power, 360 W; extraction temperature, 60 °C; and extraction time, 20 min. [Conclusion] The method is reasonable and feasible to extract effective ingredients from Zengye Chengqi decoction.

The paper introduces recent progress in the applications of proanthocyanidin in prevention and treatment of cancer and angiocardiopathy as well as treatment of eye disease, and it also discusses the safety of proanthocyanidin in practice in order to provide a reference for further development of proanthocyanidin.

The levels of progesterone, diethylstilbestrol, testosterone and methyl testosterone in fresh eggs, hatched eggs and chicken breast eggs were determined by ultra-high performance liquid chromatography tandem mass spectrometry （UPLC-MS/MS）. And the level of progesterone was also analyzed. The results indicated that progesterone could be detected in all eggs, but its level varied largely and had no regularity in different individuals. The study provides the levels of progesterone in eggs and hatched eggs from different producing areas in China in order to provide reference for food safety.

Cyan-foot white-feather chicken is a newly-bred chicken variety in recent years. In order to better utilize their excellent germplasm characteristics, other high -quality chickens were test -crossed with the cyan -foot white -feather chicken. The produced variety was expected to contain more fine characters of both parents and even superior characters. The heterosis would be accordingly utilized in production. The experimental results showed that the test cross between the cyan-foot white-feather chicken （female parent） and Chuandong black chicken （male parent） had the best economic characters. It would be complicated to explore its genetic effects of feather color after test cross. To study the genetic effects is of important significance for enriching the theory of genetics and breeding and guiding the breeding practice.

In recent years, the pregnancy rates of yellow cattle and water buffalos in many cattle breed improvement stations of Xiangzhou County of Guangxi region were 33%-51% and 35%-55%, respectively, which indicated the pregnancy rate still needed to be increased. Human chorionic gonadotropin （HCG） can promote follicular development and ovulation in cows. Cows were intramuscularly injected with HCG during estrus and then artificially inseminated, and its effects on pregnancy rate and artificial insemination of cows were observed. The results showed that HCG can significantly increased pregnancy rate in artificially inseminated cattle as well as work efficiency.

[Objective] The study was aimed to adjust and optimize production structure of rice so as to meet the requirements of the development of aquaculture. [Method] Seventeen newly-bred domestic early long-grain rice varieties were introduced. With Zhuqing, the Anhui provincial high-yield rice variety, as control, the early long-grain non-glutinous rice varieties which had high yield, high protein and high brown rice rate and could meet the requirement of plant industry and breeding industry were selected. And supporting cultivation techniques were also studied. [Result] Zhuliangyou 819 and 99 were high-yield, high-protein and high-brown rice rate rice varieties. They could be used in demonstration and promotion of rice forage in Anhui Province. Nitrogen fertilizer could significantly increased protein content in brown rice, and the optimal proportion of nitrogen was 70%. [Conclusion] Early long-grain rice has broad applicative prospect.

Fatty acid and sodium carboxymethy cellulose were used to coat arginine. Then the stability and digestibility of the rumen- protected Arginine （RP-Arg） in digestive tract of sheep were detected by artificial saliva digestion test, nylon digestion test and duodenal juice digestion test. The results showed that the release rate of RP-Arg was increased with duration of culture. After 16 h culture, the release rates of RP-Arg in saliva, rumen and duodenum were 22.16%, 31.47% and 88.85%, respectively. These results indicated that the RP-Arg had a good stability in saliva and rumen and had a good digestibility in duodenum.

China is rich in stalks. In recent years, to use ammoniated stalks to feed animals has become an important project of FAO and the Ministry of Agriculture of China. The Qinghai-Tibetan Plateau has a large quantity of stalks, but they are mainly used as fuel, which not only wastes resources to some extent but also harms environment. To make full use of barley stalks, ammonification of barley stalks was introduced based on some investigations.

The study was aimed to evaluate the characters of ereck milkvetch material and seek effective measures to improve its fermentation quality. The fresh and air basked ereck milkvetch was used. There were three treatments including water control （CK）, sucrose （2%） and formic acid （6 mL/kg）. The ereck milkvetch was fermented for 360 d in bags at RT. The results showed that the moisture content in ereck milkvetch silage dropped from 80% to 70% after air basking. Its pH value was increased significantly （P〈0.01）, but its content of lactic acid and ammonia nitrogen was reduced significantly （P0.01）. Compared with the CK, the formic acid and sucrose significantly reduced the pH value and ammonia nitrogen content （P0.01） but significantly increased lactic acid content（P〈0.05）. In addition, the sucrose significantly increased the contents of acetic acid, propanoic acid and nitrate content （P〈0.05）. Air basking and additives had no significant impact on dry matter content and recovery, but silage fermentation reduced nitrate content in ereck milkvetch by 70%-77% as well as nitrite content.

With the rapid development of animal husbandry, it promotes development of social economy, but it also causes serious pollution in eco-environment and animal products. To effectively solve the conflicts between development and pollution, the concept and features of ecological animal husbandry were introduced. On this basis, the approaches to develop ecological animal husbandry in China were proposed.

Some natural plants and mineral medicinal materials have sedative and hypnotic effects, and most of these medicinal materials are edible and have good safety. The comparison shows that the plant medicines have better safety than the mineral herbs.

Five kinds of natural medium and five kinds of synthetic medium were selected. Each medium was dumped into 20 conicalflasks. Each media had two treatments. In the first treatment, the natural media and synthetic media were sterilized for 15 min under maintaining pressure and naturally cooled until zero pressure. In the second treatment, the natural media and synthetic media were sterilized for 15 min under maintaining pressure and then the pressure was decreased to zero by releasing steam. All sterilized media were incubated in an incubator to observe contamination. The results showed that natural depressurization is suitable for sterilization of natural medium, while releasing steam is suitable for sterilization of synthetic medium.