Abstract: In recent years,avian influenza has been the top important point in the prevention and control of animal diseases in each country.Although H9N2-subtype avian influenza virus is a kind of lowly pathogenic avian influenza virus,but it has wide infection range,it has rapid occurrence speed and can bring great losses.At present,using inactivated vaccine is the main method for preventing H9-subtype avian influenza.Although this vaccine suppressed the disease symptoms of the immunized chicken in clinic,but it could not stop the toxin removal after the infection and had certain effects on the meat quality.The research showed that HVJ-E had the function of dual immunoregulation,hoping to be applied as the immune adjuvant of the inactivated vaccine of H9-subtype avian influenza virus in clinic.