Abstract: To exhibit the progress of preparing microbial testing slip,several materials such as filter paper,micro-filtration membrane,unwoven fabric or several cold water gelling agents and their combinability were used as the carrier of culture medium to develop microbial testing slips. And their effect was evaluated with Bacillus subtilis and coliforms. It was found that excellent testing slip could be prepared by using micro-filtration membrane with other materials followed with the adjustment of culture concentration and gum dosage,or the combinability of several suitable cold water gelling agents followed with their carrier stacked together face to face. Detectable rate of the ultimate products was above 85%.