Abstract: In order to study effects of different testing factors on the error rate of Bacillus licheniformis, the sample was treated by using different dilution methods and different media under different temperatures. The result showed that different dilution methods had minimal impact on the error rate of Bacillus licheniformis, the media in the second position. But it had great effects on the error rate of Bacillus licheniformis after treatment of 80 ℃ temperature for 10 minutes.