Abstract: Eight beef cattles were divided into two groups,and they were fed with the dietary containing concentrate and corn cobs. The concentrate contained different protein source,among them DDGS was tested group and cottonseed meal was control group. The apparent digestibility of dietary nutrients was determined through digestion test by total feces collection method. Results showed that apparent digestibilities in total tract of organic matter,crude protein,neutral and acid detergent fiber were unaffected by protein sources significantly. The variation ranges of apparent digestibilities of organic matter,crude protein,neutral and acid detergent fiber in beef cattle ration were 65%～71%,60%～70%,68%～70% and 63%～65%,respectively.