Abstract: [ Objective ] The research aimed to investigate the effect of the Acetyl-CoA carboxylase inhibitor on the obesity degree of obesity mice. [Method] The obesity animal model was established and mice were randomly divided. Mice in test group was fed with Acetyl-CoA carboxylase inhibitor with the dose of 50 mg/（kg, bw-d）by intragastric administration and mice in control group was treated with deionized water by intragastric administration. The changes of the feed intake and drinking amount of mice in the administration process were recorded. Mice were anatomize after administration three weeks.The body weight and body length were determined, and Lee＇s index was calculated. The fat was weighed and the proportion of the fat around the sexual organs in the body weight of mice was calculated. The relative contention of cholesterol and triglyceride in the serum was measured. [ Result ] Acetyl-CoA carboxylase inhibitor could inhibit the weight increase of mice, reduce the obese degree of obesity mice and decrease the contents of triglyceride and cholesterol in the serum of obesity mice. [ Conclusion ] Acetyl-CoA carboxylase inhibitor had the slimming action on obesity mice, but it had no obvious effect on the normal diet of obesity mice.