Abstract: The experiment was based on the comparison of general commercial chicken and Hetian black chicken lymphocyte which was not vaccinated transformation rate,analyzing the immunity strength of Hetian black chicken and general commercial chicken,isolate peripheral blood lymphocytes by density gradient centrifugation,then incubate it in carbon dioxide cell incubator at the temperature of 37 ℃,the carbon dioxide concentration was 5 %,by some inducers such as mitotic original plant lectin（PHA）,concanavalin A（ConA）,after the induction,performance of the increasing of cell volume,metabolism,protein and nucleic acid,and ordinary commodity chicken lymphocyte transformation rate is greater than Hetian black chicken,after 72 h general commercial chicken and Hetian black chicken lymphocyte transformation was performing the diminishing level,the high and low lever of lymphocyte transformation was reflect the body＇s immune function.This experiment laid the foundation for animal immune effects of detection and cytokine preparations.