Abstract: In order to clone and express rumen microbial fibrolytic enzyme genes in a strain of Lactococcus lactis, a cellulose degrade bacterium from the rumen of the sheep was isolated by using improved CMC medium based on widely screen. The bacterium was identified as Staphylococcus epidermidis by using analysis of morphology and the conserved sequence 16S rDNA. Two β-glucanase genes were cloned from the bacterium chromosome DNA by original strain of Staphylococcus epidermidis. The two β-glucanase genes were connected with vector pMG36e to construct β-glucanase gene expression vector, and then transformed into E. coli and Lactococcus lactis MG1363. Both them could express β-glucanase, and their enzyme activities reached 1.47 U/mL and 1.54 U/mL respectively, which were higher 1.49 times and 1.56 times than that of original strain of Staphylococcus epidermidis （0.99 U/mL）.