Abstract: In this study, the CDS sequence of the bovine HSL gene was cloned and bioinformatically analyzed, and its tissue expression profile was characterized. One set of primers was designed based on the bovine HSL gene sequence （NM_001080220） registered in the GenBank, and the CDS sequence of HSL gene in bovine tissue samples was amplified using RT-PCR assay and was determined by sequencing; the expression level of HSL gene in bovine tissues was detected by semi-quantitative RT-PCR;the HSL protein encoded by HSL gene was bioinformatically analyzed. The results showed that the obtained CDS sequence of bovine HSL gene was 2 271 bp in length encoding 756 amino acids, and it shared the highest nucleotide homology （99.9%） with the bovine HSL gene registered in the GenBank; the HSL protein was hydrophobic and contained a HSL-N superfamily conserved domain with no signal peptide structure; semi-quantitative RT-PCR revealed that the HSL gene was expressed in 8 tissues including heart, spleen, lung, kidney, liver, greater omentum, subcutaneous fat and muscle; the highest expression level was observed in the greater omentum and subcutaneous fat, and the moderate expression level was found in kidney, spleen, liver, lung, muscle and heart. This study may provide a reference for assessing the structure and function of bovine HSL proteins.

Key words: cloning, analysis, HSL gene, bioinformatics, expression profile, bovine