Abstract: [Objective] Effects of sheep placental transfer factor injection （SFFF）on immunoglobulin, B lymphocyte and antibody forming cell of tested animals were studied, and the immune regulation of SPIT in animals were explored to evaluate the use value. [ Method ] In test 1, mice were used for the detection of serum immunoglobulin, and the changes of serum immunoglobulin of mice pre- and post injection were compared. In test 2, rabbits were used for EAC rosette test, and the changes of B lymphocyte of mice pre- and post injection were compared. In test 3, mice were used for hemolytic plaque assay, and the changes of antibody forming cell of mice pre- and post injection were compared. [Result] In test 1, SPTF significantly improved the serum immunoglobulin in test group. In test 2, the numbers of EAC rosette forming cells in test group were significantly higher than control. In test 3, the numbers of antibody forming cell of tested animals were very significantly increased. [ Conclusion ] SPTF has significant humoral immune regulation effect on animals.