Abstract: Brucellosis was one of the important zoonosis caused by different kinds of Brucella spp. The most common clinical manifestations of the infected animals were abortion,placentitis,epididymitis and orchitis. In human being,brucellosis was a debilitating and chronic disease, which could injure a variety of organs. Although etiology identification was a conventional and effective diagnosis method, but it was strict with personal safety and requirements of laboratory. The process of isolating pathogen from infected animal was time-consuming and hazardous,so it was difficult to be popularized in the clinical diagnosis of brucellosis. The serological diagnosis method showed good specificity and sensibility,but it was hard to identify various wild strains and the infection of vaccine strain. In view of the above factors, it was necessary to in-depth develop new diagnosis method of brucellosis with better specificity, sensibility and stability in the veterinary clinical practice. The diagnosis methods of brucellosis were reviewed from the bacteriological, serological and molecular biology aspects, so as to provide references for the related studies.