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Animal Husbandry and Feed Science ›› 2014, Vol. 35 ›› Issue (1): 12-12.doi: 10.12160/j.issn.1672-5190.2014.01.007

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Preparation of Polyclonal Antibodies of Mannanase and the Establishment of ELISA Double-antibody Sandwich Method

FAN Wen-bin, WANG He-ping, LIU Yong-bin （1.Hohhot Vocational College, Hohhot 010051 ,China;2.Inner Mongolia Agricultural University,Hohhot 010018,China;3. Inner Mongolia Academy of Agricultural ＆ Animal Husbandry Sciences, Hohhot 010031, China）   

  • Online:2014-01-20 Published:2014-01-20

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Abstract: The purpose of this study was to establish an effective method for detecting the expressed product by Escherichia coli transformed byβ-mannanase. Due to little expression amount and the changes of product structure, the conventional detection method couldn′t accurately judge the properties of expression product. So the selfmade antigen and anti-serum were used to establish ELISA. The detection results showed that E. coli transformed byβ-mannanase could successfully expressβ-mannanase.

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