Abstract: In order to assess the nutritional components of eggs from different breeds of laying hens, forty eggs from each of the three breeds of laying hens aged 43 weeks, including Lhasa White Chicken, Tibetan Chicken and Liuhongguxiang Chicken, were chosen and used to determine their nutrition constituents. The results showed that except for protein, 16 amino acids and fatty acid （C18∶3） （P〉0.05）, there were differences in other nutrition components between eggs from the three breeds of laying hens. Significant differences in moisture content and （C14∶0） content between Lhasa White Chicken eggs, Tibetan Chicken eggs and Liuhongguxiang Chicken eggs were observed （P〈0.05）;there were extreme significant differences in content of C17∶0, C22∶6 as well as mineral elements of Mg and Na between Lhasa White Chicken eggs, Liuhongguxiang Chicken eggs and Tibetan Chicken eggs （P〈0.01）;extreme significant differences in content of fat, C16∶0, C16∶1, C18∶0, C18∶1, C18∶2, C19∶0 as well as mineral elements of Zn, Cu, Fe, Mn, Ca between the eggs from the three breeds of laying hens were observed （P〈0.01）;the content of mineral element of K between the eggs from Lhasa White Chicken, Tibetan Chicken and Liuhongguxiang Chicken was mutually extremely significantly different （P〈0.01）;the content of phospholipid, cholesterin and mineral element of P between the eggs from the three breeds of laying hens was mutually extremely significantly different or significantly different （P〈0.05 or P〈0.01）.