Abstract: In order to explore the hereditary adaptability of high altitude ecological environment of Tibetan chickens, this study compared the difference among Tibetan chickens from different areas （Tibet, Ganzi, and low altitude area）,The blood RBC, HGB, and MCV levels were higher in Tibetan chickens in Tibet than those in Ganzi （P〈0.05）,meanwhile the RBC, HGB, and MCV concentrations were greater in Tibetan chicken in Ganzi than that in low altitude area （P〈0.05）. Tibetan chickens in low altitude area had the highest MCH and MCHC levels among three kinds of Tibetan chicken （P〈O.05）,these criteria were decreased in the Tibetan chickens in Tibet compared with those in Ganzi （P〈0.05）. There was an increasing trend in the RDW-SD in Tibetan chickens as the altitude increased. The Tibetan chickens in high altitude area had greater blood total protein,albumin,globulin, glucose and total cholesterol concentrations than those in low altitude area （P〈0.01）.The concentrations of triglyceride,total cholesterol,and alkaline phosphatase were greater in Tibetan chickens in Ganzi than those in low altitude area （P〈0.01）.In conclusion,the Tibetan chickens living in 3 800 m altitude and 1 000 m altitude after 9 years of cultivation have great difference in physiological and biochemical indicators such as RBC, HGB, MCV, RDW-SD, MCH, MCHC, TP, ALB, GLO, GLU, and TC.