无量山乌骨鸡脂联素基因的多态性分析

doi:10.12160/j.issn.1672-5190.2020.05.012

Abstract

Abstract: In this study, a total of 208 individuals of Wuliangshan black-bone chicken were chosen to screen the mutations of c.A99G locus in exonⅠdigested by Hsp92Ⅱand the mutations of c.C565G locus in exonⅡdigested by MvaⅠin the adiponectin gene （accession number NC_006096） via polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP）. There were three genotypes （AA, AB and BB） in c.A99G locus and three genotypes （CC, CD and DD） in c.C565G locus. Analysis of the population genetic characteristics showed that the information content of the peptides at the c.A99G locus was at moderate-polymorphic level, while that of the peptides at the c.C565G locus was at low-polymorphic level. The Chi-square （χ²） fitness test demonstrated that both loci of c.A99G and c.C565G of the population were in equilibrium state.

Key words: Wuliangshan black-bone chicken, adiponectin gene, PCR-RFLP

CLC Number:

S831.892

TANG Tian-tian, DING Xiang-hong, CHEN Fen-fen. Polymorphic Analysis of Adiponectin Gene in Wuliangshan Black-bone Chicken[J]. Animal Husbandry and Feed Science, 2020, 41(5): 63-66.

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Polymorphic Analysis of Adiponectin Gene in Wuliangshan Black-bone Chicken

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