Animal Husbandry and Feed Science ›› 2018, Vol. 39 ›› Issue (7): 31-31.doi: 10.12160/j.issn.1672-5190.2018.07.007

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Construction of Recombinant Eukaryotic Expression Vector of pEGFP-N3-UCP3 in Guanling Cattle

DAI Zhen-xin[1];CHEN Wei[1,2,3,4];HAN Xue[5];XU Hou-qiang[1,2,3];WU Guo-wen[1];SHEN Yong[1];XIONG Yuan-song[1]([1]College of Animal Science,Guizhou University,Guiyang 550025,China;[2]Key Laboratory of Animal Genetics,Breeding and Reproduction in the Plateau Mountainous Region,Ministry of Education,Guiyang 550025,China;[3]Guizhou Key Laboratory of Animal Genetics,Breeding and Reproduction,Guiyang 550025,China;[4]College of Life Science,Guizhou University,Guiyang 550025,China;[5]Institute of Animal Husbandry and Veterinary Medicine,Guizhou Academy of Agricultural Sciences,Guiyang 550025,China)   

  • Online:2018-07-20 Published:2018-07-20

Abstract: This study was carried out to construct a recombinant eukaryotic expression vector of pEGFP-N3-UCP3 without CMV promoter.UCP3 gene promoter and pEGFP-N3 vector fragment without CMV region was cloned and amplified by PCR assay.Subsequently,the obtained UCP3 gene promoter and the amplified vector were connected and transformed into competent cell DH5αfrom Escherichia coli.The positive clone was selected and identified by PCR assay and sequencing.The recombinant eukaryotic expression vector of pEGFP-N3-UCP3 was successfully constructed,which laid a foundation for uncovering the mechanism underlining the transcriptional regulation of UCP3 gene.

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