Abstract: This review aimed to provide references for the investigation of animal manure treatment technology by using earthworms. Scientific literatures demonstrated that the variety selection of earthworms, environmental elements, maturity of animal manure, combination of different animal manure, and excipient composition are primary influencing factors associated with animal manure treatment effect using earthworms. Eisenia are the most commonly investigated and used earthworm variety at home and abroad; the treatment temperature is recommended to be maintained at 15 to 25℃, and the pH value are expected to be controlled at the neutral range; the earthworms have best growth performance in cattle manure; accordingly, to improve the manure treatment effect, the cattle manure with dominant ratio is recommended to be supplemented in other animals＇ the manure. The impact of humidity, manure maturity as well as excipient selection and composition under different treatment conditions on animal manure treatment effect are necessary to be further studied.